Overview

  • Product nameAnti-F4/80 antibody [BM8] (FITC)
    See all F4/80 primary antibodies
  • Description
    Rat monoclonal [BM8] to F4/80 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis antibody reacts with a 125kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. It does not cross react with any of the following mouse cell types: Granulocytes, mast cells, platelets, lymophocytes, fibroblasts or endothelial cells. Although several publications have used this antibody succesfully in human, we have been unable to obtain positive results in this species and so do not guarantee it.
  • Tested applicationsSuitable for: WB, IHC-Fr, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.

  • Positive control
    • Mouse macrophages.
  • General notes


    This antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. It is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purification notesPurity: 0.2µm filtered.
  • Primary antibody notesThis antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. It is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.
  • ClonalityMonoclonal
  • Clone numberBM8
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab60343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 98 kDa.
IHC-Fr 1/50. tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT; incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control.
Flow Cyt 1/50 - 1/100. ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P 1/50. fixation in 10% neutral buffered formalin for 24 h; blocking with non-immunized goat serum; microwaved for 6 min in citrate buffer; splenic macrophages as positive control.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionOrphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificityExpression is restricted to eosinophils.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [BM8] (FITC) images

  • ICC/IF image of ab60343 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1µg/ml, FITC conjugated) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing RAW 264.7 cells stained with ab60343 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1/100 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) with secondary antibody DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a minimal signal in RAW 264.7 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-F4/80 antibody [BM8] (FITC) (ab60343)

This product has been referenced in:
  • Balamurugan K  et al. FBXW7a attenuates inflammatory signalling by downregulating C/EBPd and its target gene Tlr4. Nat Commun 4:1662 (2013). Read more (PubMed: 23575666) »
  • Majumder M  et al. A practical and sensitive method of quantitating lymphangiogenesis in vivo. Lab Invest 93:779-91 (2013). Mouse . Read more (PubMed: 23711825) »

See all 8 Publications for this product

Product Wall

Thank you for contacting us.

Antibody ab60343 is a well-characterized F4/80 clone, BM8. If the cells express F4/80, the antibody will detect it. For more references, see the datasheet for the unconjugated version, ab16911:

Click h...

Read More

Thank you for your enquiry.

We do not have expire dates for our products and you should be able to use the product without anydifficulty if kept at the recommend storage temperature for at least one year. Please rest assured that our "A...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"