Overview

  • Product name
    Anti-F4/80 antibody - C-terminal
    See all F4/80 primary antibodies
  • Description
    Chicken polyclonal to F4/80 - C-terminal
  • Host species
    Chicken
  • Tested applications
    Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse F4/80 (C terminal).
    Database link: Q61549

  • Positive control
    • Mouse spleen lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 98% PBS, 1% Trehalose
  • Concentration information loading...
  • Purity
    IgY fraction
  • Purification notes
    Crude antiserum was purified by Thiopropyl Sepharose affinity chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab186073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 102 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity
    Expression is restricted to eosinophils.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Images

  • Anti-F4/80 antibody - C-terminal (ab186073) at 1 µg/ml + Mouse spleen lysate

    Developed using the ECL technique.

    Predicted band size: 102 kDa


    Exposure time: 5 seconds
  • Chicken anti mouse F4/80 (ab186073, polyclonal) staining of mouse RAW cells by immunofluorescence, using a FITC-conjugated anti chicken antibody as a secondary antibody.

  • Immunohistochemistry using Chicken anti-F4/80 (ab186073, polyclonal) on mouse spleen tissue, showing staining of macrophages in the germinal center and perifollicular zone. DAB peroxidase was used for detection.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (gastrocnemius muscle)
Permeabilization
No
Specification
gastrocnemius muscle
Blocking step
Dako / Protein Block / X0909 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
nothing
Username

Abcam user community

Verified customer

Submitted Nov 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 04 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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