Overview

  • Product nameAnti-F4/80 antibody [CI:A3-1]
    See all F4/80 primary antibodies
  • Description
    Rat monoclonal [CI:A3-1] to F4/80
  • SpecificityThis antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.
  • Tested applicationsWB, IP, IHC-P, RIA, IHC-Fr, Flow Cyt, ICC, IHC-FoFr, ICC/IF, IHC-Rmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Thioglycollate stimulated peritoneal macrophages from C57/BL mice

  • Positive control
    • Mouse spleen and macrophages

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality Monoclonal
  • Clone numberCI:A3-1
  • MyelomaNS1
  • IsotypeIgG2b
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Use under non reducing condition. Detects a band of approximately 160 kDa (predicted molecular weight: 102 kDa). (predicted molecular weight of precursor protein: 102 kDa; protein is heavily glycosylated). Block in 5% milk for 1 hour.
IP Use at an assay dependent dilution.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval (e.g. with citrate buffer) or carry out Proteinase K pre-treatment (starting treatment time 3min).
RIA Use at an assay dependent dilution.
IHC-Fr Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent dilution.
ICC Use at an assay dependent concentration.
IHC-FoFr Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent dilution.
IHC-R Use a concentration of 10 µg/ml.

Target

  • FunctionCould be involved in cell-cell interactions.
  • Tissue specificityWide expression; increased levels in peripheral blood mononuclear cells.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 antibody
    • EMR1 hormone receptor antibody
    • EMR1_HUMAN antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [CI:A3-1] images

  • ab6640 staining F4/80 (red) in Mouse intestine tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/10µg/ml in PBS + 10% serum) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rat IgG polyclonal (1/1000) was used as the secondary antibody. Blue - nuclei.

    See Abreview

  • ab6640 staining F4/80 in Mouse skeletal muscle tissue by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to Heat-mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1% BSA for 25 minutes at 25°C. The primary antibody was diluted 1/100 in 1%BSA/PBS and incubated with the sample for 1 hour 30 minutes at 25°C. An HRP polymer-conjugated Goat anti-Rat polyclonal was used as the secondary antibody.

    See Abreview

  • ab6640 staining mouse brain tissue sections by IHC-FoFr.  Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C.  The primary antibody was diluted 1/10 and incubated with the sample for 18 hours at 4°C.  A biotinylated goat anti-rat antibody, diluted 1/500, was used as the secondary.  This image demonstrates quiescent microglia in the normal mouse brain (red).

    See Abreview

  • ab6640 staining F4/80 in mouse adipose tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation and blocking with 5% serum was performed for 20 minutes at 20°C. The primary antibody was diluted 1/150 and incubated with sample in Tris + 5 % normal rabbit serum for 1 hour at 20°C. A Biotin conjugated rabbit polyclonal to rat IgG was used at dilution at 1/50 as secondary antibody. The antibody stained interstitial macrophages in the adipose tissue of lean mice (A), and macrophage associated with "crown-like structure" in mice fed high-fat diet (B).

    See Abreview

  • ab6640 staining F4/80 in mouse bone marrow cells by immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hour at 4°C. Alexa fluor® 488 goat polyclonal to rat Ig, diluted 1/200, was used as the secondary antibody.

    See Abreview

  • Anti-F4/80 antibody [CI:A3-1] (ab6640) at 1/1000 dilution (for 8 hours at 4°C) + Mouse pancreatic cancer whole tissue lysate. at 25 µg

    Secondary
    An HRP-conjugated Goat anti-rat IgG polyclonal at 1/5000 dilution
    developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : ~130 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    This image is courtesy of an Abreview submitted by Pawel Mazur

    Blocking Step: 10% Milk for 1 hour at room temperature

    See Abreview

References for Anti-F4/80 antibody [CI:A3-1] (ab6640)

This product has been referenced in:
  • O'Meara CP  et al. Immunity against a Chlamydia infection and disease may be determined by a balance of IL-17 signaling. Immunol Cell Biol 92:287-97 (2014). Read more (PubMed: 24366518) »
  • Christensen DP  et al. Lysine deacetylase inhibition prevents diabetes by chromatin-independent immunoregulation and ß-cell protection. Proc Natl Acad Sci U S A 111:1055-9 (2014). Mouse . Read more (PubMed: 24395784) »

See all 86 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample Mouse Tissue sections (Breast tumor)
Specification Breast tumor
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Gajendra Katara

Verified customer

Submitted Sep 05 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample Mouse Cell (Peritoneal macrophages)
Specification Peritoneal macrophages
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 19 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Cell (peritoneal macrophage)
Specification peritoneal macrophage
Permeabilization Yes - 0.2% Triton X100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 19 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Heart)
Specification Heart
Permeabilization No
Fixative Formalin 10%
Username

Abcam user community

Verified customer

Submitted Jul 21 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteinaseK
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - triton-x
Fixative Paraformaldehyde
Username

Miss. Cathy Wyse

Verified customer

Submitted May 28 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample Mouse Tissue sections (adipose tissue)
Specification adipose tissue
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 19 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Sample Mouse Tissue sections (uterus)
Specification uterus
Permeabilization No
Fixative Formaldehyde
Username

Dr. Varkha Agrawal

Verified customer

Submitted Nov 14 2013

Application Immunocytochemistry
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Sample Mouse Cell (Testis macrophage)
Specification Testis macrophage
Permeabilization Yes - 3% BSA in PBS with 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 12 2013

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Sample Mouse Tissue sections (mouse intestine)
Specification mouse intestine
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

Submitted Oct 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Sample Mouse Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"