Overview

  • Product name
    Anti-F4/80 antibody [CI:A3-1] (FITC)
    See all F4/80 primary antibodies
  • Description
    Rat monoclonal [CI:A3-1] to F4/80 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab105155 recognises the murine F4/80 antigen.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Thioglycollate stimulated peritoneal macrophages from C57/BL mice.

  • Positive control
    • Mouse peritoneal macrophages
  • General notes


    Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.
  • Clonality
    Monoclonal
  • Clone number
    CI:A3-1
  • Myeloma
    NS1
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. in 100ul.



ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity
    Expression is restricted to eosinophils.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [CI:A3-1] (FITC) images

  • Flow cytometry staining of mouse peritoneal macrophages with ab105155.
  • FSC/SSC gated mouse peritoneal macrophages dual stained with ab105155 at 1/5 dilution and anti-CD11b at 1/5 dilution by Flow cytometry. Isotype control pair in red. Fc receptors were blocked.

References for Anti-F4/80 antibody [CI:A3-1] (FITC) (ab105155)

This product has been referenced in:
  • Taylor L  et al. Netrin-1 Reduces Monocyte and Macrophage Chemotaxis towards the Complement Component C5a. PLoS One 11:e0160685 (2016). Read more (PubMed: 27509208) »
  • Suárez-Zamorano N  et al. Microbiota depletion promotes browning of white adipose tissue and reduces obesity. Nat Med 21:1497-1501 (2015). Flow Cyt ; Mouse . Read more (PubMed: 26569380) »

See all 2 Publications for this product

Product Wall

A suitable isotype control for ab105155 is product ab136125: Rat IgG2b, kappa monoclonal (FITC) - Isotype Control.

The light chains of antibody ab105155 are kappa isotype.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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