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Thioglycollate stimulated peritoneal macrophages from C57/BL mice.
Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.
Our Abpromise guarantee covers the use of ab105155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells. in 100ul.
ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FSC/SSC gated mouse peritoneal macrophages dual stained with ab105155 at 1/5 dilution and anti-CD11b at 1/5 dilution by Flow cytometry. Isotype control pair in red. Fc receptors were blocked.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"