Overview

  • Product name
    Anti-F4/80 antibody [SP115]
    See all F4/80 primary antibodies
  • Description
    Rabbit monoclonal [SP115] to F4/80
  • Tested applications
    Suitable for: IHC-P, IHC-FrFlmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from the N terminus of Mouse F4/80 protein.

  • Positive control
    • Mouse liver tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab111101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

For antigen retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min.

IHC-FrFl Use at an assay dependent concentration. PubMed: 24647450

Target

  • Function
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity
    Expression is restricted to eosinophils.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [SP115] images

  • ab111101 staining F4/80 in Mouse uterus and spleen (multi block) tissue sections

    See Abreview

  • ab111101 at 1/100 dilution staining F4/80 in Formalin-fixed, paraffin-embedded Mouse liver tissue.

  • Immunohistochemistry analysis of Formalin fixed paraffin-embedded mouse lung tissue sections labeling F4/80 with ab111101 at 1/200 for 16 hours at 4°C. Biotin conjugated Goat anti-rabbit polyclonal antibody at 1/500 was used as the secondary. Antigen retrieval was heat mediated using citrate buffer pH 6.0. 

    See Abreview

References for Anti-F4/80 antibody [SP115] (ab111101)

This product has been referenced in:
  • Li X  et al. Fructus xanthii improves lipid homeostasis in the epididymal adipose tissue of rats fed a high-fat diet. Mol Med Rep 13:787-95 (2016). IHC . Read more (PubMed: 26648271) »
  • Akashi K  et al. Knockout of endothelin type B receptor signaling attenuates bleomycin-induced skin sclerosis in mice. Arthritis Res Ther 18:113 (2016). IHC-P ; Mouse . Read more (PubMed: 27209208) »

See all 7 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (uterus and spleen (multi-block))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako high pH buffer
Permeabilization
No
Specification
uterus and spleen (multi-block)
Blocking step
Vector casein solution as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Henry King

Verified customer

Submitted Aug 07 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
Fixative
10% NBF
Username

Abcam user community

Verified customer

Submitted Jul 11 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (rat blood vessels)
Antigen retrieval step
None
Permeabilization
No
Specification
rat blood vessels
Blocking step
Dako blocking solution as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 01 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung)
Permeabilization
No
Specification
lung
Fixative
Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

Submitted Dec 01 2016

Application
Flow Cytometry
Sample
Mouse Cell (Leukocytes from lung (bronchoalveolar lavage))
Permeabilization
No
Gating Strategy
CD45+ cells
Specification
Leukocytes from lung (bronchoalveolar lavage)
Preparation
Cell harvesting/tissue preparation method: Bronchoaveolar lavage in balb/c mice was performed with PBS-EDTA 3mM
Sample buffer: PBS-EDTA 3mM
Fixation
None, cells were used fresh
Username

Dr. Francois Daubeuf

Verified customer

Submitted Dec 01 2016

Application
Immunocytochemistry
Sample
Mouse Cell (Leukocytes from lung (bronchoalveolar lavage))
Permeabilization
No
Specification
Leukocytes from lung (bronchoalveolar lavage)
Fixative
Dehydrated cells by Cytospin
Username

Dr. Francois Daubeuf

Verified customer

Submitted Dec 01 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph=6
Permeabilization
No
Specification
lung
Fixative
Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

Submitted Dec 01 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Macrophages from Bronchoalveolar lavage)
Permeabilization
No
Specification
Macrophages from Bronchoalveolar lavage
Fixative
None, cells were used fresh
Username

Dr. Francois Daubeuf

Verified customer

Submitted Nov 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 23 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Infarcted mouse heart)
Specification
Infarcted mouse heart
Permeabilization
No
Fixative
Acetone
Username

Nick

Verified customer

Submitted Jul 15 2014

1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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