Overview

  • Product nameAnti-F4/80 antibody [SP115]
    See all F4/80 primary antibodies
  • Description
    Rabbit monoclonal [SP115] to F4/80
  • Tested applicationsSuitable for: IHC-P, IHC-FrFlmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from the N terminus of Mouse F4/80 protein.

  • Positive control
    • Mouse liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab111101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

For antigen retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min. This product is sensitive to tissue ageing and use of fresh cut sections is recommended.

IHC-FrFl Use at an assay dependent concentration. PubMed: 24647450

Target

  • FunctionOrphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificityExpression is restricted to eosinophils.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [SP115] images

  • ab111101 at 1/100 dilution staining F4/80 in Formalin-fixed, paraffin-embedded Mouse liver tissue.

  • Immunohistochemistry analysis of Formalin fixed paraffin-embedded mouse lung tissue sections labeling F4/80 with ab111101 at 1/200 for 16 hours at 4°C. Biotin conjugated Goat anti-rabbit polyclonal antibody at 1/500 was used as the secondary. Antigen retrieval was heat mediated using citrate buffer pH 6.0. 

    See Abreview

References for Anti-F4/80 antibody [SP115] (ab111101)

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (lung)
Permeabilization No
Specification lung
Fixative Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

Submitted Dec 01 2016

Application Flow Cytometry
Sample Mouse Cell (Leukocytes from lung (bronchoalveolar lavage))
Permeabilization No
Gating Strategy CD45+ cells
Specification Leukocytes from lung (bronchoalveolar lavage)
Preparation Cell harvesting/tissue preparation method: Bronchoaveolar lavage in balb/c mice was performed with PBS-EDTA 3mM
Sample buffer: PBS-EDTA 3mM
Fixation None, cells were used fresh
Username

Dr. Francois Daubeuf

Verified customer

Submitted Dec 01 2016

Application Immunocytochemistry
Sample Mouse Cell (Leukocytes from lung (bronchoalveolar lavage))
Permeabilization No
Specification Leukocytes from lung (bronchoalveolar lavage)
Fixative Dehydrated cells by Cytospin
Username

Dr. Francois Daubeuf

Verified customer

Submitted Dec 01 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer ph=6
Permeabilization No
Specification lung
Fixative Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

Submitted Dec 01 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Macrophages from Bronchoalveolar lavage)
Permeabilization No
Specification Macrophages from Bronchoalveolar lavage
Fixative None, cells were used fresh
Username

Dr. Francois Daubeuf

Verified customer

Submitted Nov 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
Permeabilization No
Specification spleen
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 23 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step None
Sample Mouse Tissue sections (Infarcted mouse heart)
Specification Infarcted mouse heart
Permeabilization No
Fixative Acetone
Username

Nick

Verified customer

Submitted Jul 15 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Mouse Tissue sections (bone)
Specification bone
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 24 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
Sample Mouse Tissue sections (Liver)
Specification Liver
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 16 2013


The recommended antigen retrieval buffer is 1 mM EDTA solution, pH 8, in water.

1-10 of 17 Abreviews or Q&A

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