Overview

  • Product name
    Anti-FA20A antibody
  • Description
    Rabbit polyclonal to FA20A
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 55-85 of Human FA20A, conjugated to KLH.

  • Positive control
    • MCF7 cell lysate; Human breast carcinoma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 62 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    FA20A belongs to the FAM20 family. All FAM20 proteins contain putative conserved signal sequences as well as a conserved C terminal domain. FA20A is a secreted glycoprotein. It has been found in EML and MPRO cell lines, with low levels in undifferentiated cells. FA20A is induced during maturation to promyelocyte stage of neutrophil differentiation and decreased during neutrophil terminal differentiation.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Family with sequence similarity 20, member A antibody
    • FP2747 antibody

Images

  • Anti-FA20A antibody (ab104078) at 1/100 dilution + MCF7 cell lysate at 35 µg

    Predicted band size : 62 kDa
  • ab104078, at a 1/50 dilution, staining FA20A in Human breast carcinoma by Immunohistochemistry, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab104078 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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I have copied below the testing WB protocol for this antibody, including sample preparation. I hope this will be helpful to you. Please note that we provide this as a guideline only, and further i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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