Overview

  • Product name
  • Description
    Rabbit polyclonal to FA2H
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Monkey
  • Immunogen

    Synthetic peptide within Human FA2H aa 127-157 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q7L5A8

  • Positive control
    • ZR-75-1 cell line lysates; mouse brain tissue lysates; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab175093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 43 kDa.

Target

  • Function
    Required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids.
  • Tissue specificity
    Detected in differentiating cultured keratinocytes (at protein level). Detected in epidermis and cultured keratinocytes. Highly expressed in brain and colon. Detected at lower levels in testis, prostate, pancreas and kidney.
  • Involvement in disease
    Defects in FA2H are the cause of leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia (DLDSP) [MIM:612443]. The disorder consists of a progressive neurologic disease manifested by spasticity, disordered tonicity of muscle, and white matter degeneration.
    Defects in FA2H are a cause of spastic paraplegia autosomal recessive type 35 (SPG35) [MIM:612319]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
  • Sequence similarities
    Belongs to the sterol desaturase family. SCS7 subfamily.
    Contains 1 cytochrome b5 heme-binding domain.
  • Domain
    The histidine box domains may contain the active site and/or be involved in metal ion binding.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FA2H antibody
    • FA2H_HUMAN antibody
    • FAAH antibody
    • FAH1 antibody
    • Fatty acid 2 hydroxylase antibody
    • Fatty acid 2-hydroxylase antibody
    • Fatty acid alpha hydroxylase antibody
    • Fatty acid alpha-hydroxylase antibody
    • Fatty acid hydroxylase domain containing 1 antibody
    • FAXDC1 antibody
    • FLJ25287 antibody
    • SCS7 antibody
    • Spastic paraplegia 35 (autosomal recessive) antibody
    • SPG35 antibody
    see all

Images

  • Anti-FA2H antibody (ab175093) at 1/100 dilution + ZR-75-1 cell line lysate at 35 µg

    Predicted band size : 43 kDa
  • Anti-Folylpolyglutamate synthase antibody (ab75093) at 1/100 dilution + mouse brain tissue lysate at 35 µg

    Predicted band size : 43 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded brain tissue labeling FA2H with ab175093 at 1/50 dilution. Detection utilised peroxidase conjugation of the secondary antibody and DAB staining.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175093.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up