Overview

  • Product nameAnti-FA2H antibody
    See all FA2H primary antibodies
  • Description
    Mouse polyclonal to FA2H
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human FA2H protein (AAH17049.2)

  • Positive control
    • FA2H transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab68902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionRequired for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids.
  • Tissue specificityDetected in differentiating cultured keratinocytes (at protein level). Detected in epidermis and cultured keratinocytes. Highly expressed in brain and colon. Detected at lower levels in testis, prostate, pancreas and kidney.
  • Involvement in diseaseDefects in FA2H are the cause of leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia (DLDSP) [MIM:612443]. The disorder consists of a progressive neurologic disease manifested by spasticity, disordered tonicity of muscle, and white matter degeneration.
    Defects in FA2H are a cause of spastic paraplegia autosomal recessive type 35 (SPG35) [MIM:612319]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
  • Sequence similaritiesBelongs to the sterol desaturase family. SCS7 subfamily.
    Contains 1 cytochrome b5 heme-binding domain.
  • DomainThe histidine box domains may contain the active site and/or be involved in metal ion binding.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FA2H antibody
    • FA2H_HUMAN antibody
    • FAAH antibody
    • FAH1 antibody
    • Fatty acid 2 hydroxylase antibody
    • Fatty acid 2-hydroxylase antibody
    • Fatty acid alpha hydroxylase antibody
    • Fatty acid alpha-hydroxylase antibody
    • Fatty acid hydroxylase domain containing 1 antibody
    • FAXDC1 antibody
    • FLJ25287 antibody
    • SCS7 antibody
    • Spastic paraplegia 35 (autosomal recessive) antibody
    • SPG35 antibody
    see all

Anti-FA2H antibody images

  • All lanes : Anti-FA2H antibody (ab68902) at 1/500 dilution

    Lane 1 : FA2H transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 43 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-FA2H antibody (ab68902)

This product has been referenced in:
  • Palumbo-Zerr K  et al. Inactivation of fatty acid amide hydrolase exacerbates experimental fibrosis by enhanced endocannabinoid-mediated activation of CB1. Ann Rheum Dis 71:2051-4 (2012). Read more (PubMed: 22915616) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hamster CHO cells transfected with human FA2H cDNA)
Specification Hamster CHO cells transfected with human FA2H cDNA
Fixative Formaldehyde
Permeabilization Yes - 0.5% Nonidet P-40
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted Apr 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"