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Product name
Anti-FAAH1 antibody
See all FAAH1 products (5) ...
Description
Mouse monoclonal to FAAH1
Tested applications
WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant fragment: DLNAPGRATG AVSYTMLYNC LDFPAGVVPV TTVTAEDEAQ MEHYRGYFGD IWDKMLQKGM KKSVGLPVAV QCVALPWQEE LCLRFMREVE RLMTPEKQSS , corresponding to amino acids 480-580 of Human FAAH
DLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQ MEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEE LCLRFMREVERLMTPEKQSS
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Signal Transduction >> Metabolism >> Lipid metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 63 kDa.
IP: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution. See Abreview.
Target
Function
Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.
Tissue specificity
Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.
Sequence similarities
Belongs to the amidase family.
Cellular localization
Endomembrane system. Cytoplasm > cytoskeleton. Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.
Target information above from: UniProt accessionO00519
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Anandamide amidohydrolase 1 antibody
- Anandamide amidohydrolase antibody
- FA2H antibody
see all
Database links
- Entrez Gene: 2166 Human
- Entrez Gene: 14073 Mouse
- Entrez Gene: 29347 Rat
- Omim: 602935 Human
- SwissProt: O00519 Human
- SwissProt: O08914 Mouse
- SwissProt: Q5BKA3 Rat
- SwissProt: P97612 Rat
see all
Anti-FAAH1 antibody images:
Immunoprecipitation - Anti-FAAH1 antibody (ab54615)
FAAH1 was immunoprecipitated using 0.5mg Mouse Liver whole tissue extract, 5µg of Mouse monoclonal to FAAH1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab54615.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 63kDa: FAAH1.
Western blot - Anti-FAAH1 antibody (ab54615)
Predicted band size : 63 kDa
FAAH antibody (ab54615) at 1ug/lane + A-431 cell lysate at 25ug/lane.
Western blot - Anti-FAAH1 antibody (ab54615)
Anti-FAAH1 antibody (ab54615) + Rat Brain
Predicted band size : 63 kDa
Western blot - Anti-FAAH1 antibody (ab54615)
Anti-FAAH1 antibody (ab54615) + PC-12
Predicted band size : 63 kDa
Western blot - Anti-FAAH1 antibody (ab54615)
Anti-FAAH1 antibody (ab54615) + NIH/3T3
Predicted band size : 63 kDa
References for Anti-FAAH1 antibody (ab54615)
ab54615 has not yet been referenced specifically in any publications.
Publishing research using ab54615? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"