Overview

  • Product nameAnti-FAAH1 antibody
    See all FAAH1 primary antibodies
  • Description
    Mouse monoclonal to FAAH1
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human FAAH1 aa 480-579.
    Sequence:

    DLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGD IWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS

Properties

Applications

Our Abpromise guarantee covers the use of ab54615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24223930
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 63 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. See Abreview.

Target

  • FunctionDegrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.
  • Tissue specificityHighly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.
  • Sequence similaritiesBelongs to the amidase family.
  • Cellular localizationEndomembrane system. Cytoplasm > cytoskeleton. Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anandamide amidohydrolase 1 antibody
    • Anandamide amidohydrolase antibody
    • FA2H antibody
    • FAAH antibody
    • FAAH-1 antibody
    • FAAH1_HUMAN antibody
    • Fatty acid 2-hydroxylase antibody
    • Fatty acid alpha-hydroxylase antibody
    • Fatty acid amide hydrolase antibody
    • Fatty-acid amide hydrolase 1 antibody
    • MGC102823 antibody
    • MGC138146 antibody
    • Oleamide hydrolase 1 antibody
    • Oleamide hydrolase antibody
    see all

Anti-FAAH1 antibody images

  • FAAH1 was immunoprecipitated using 0.5mg Mouse Liver whole tissue extract, 5µg of Mouse monoclonal to FAAH1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab54615.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 63kDa: FAAH1.


  • Predicted band size : 63 kDa
  • Anti-FAAH1 antibody (ab54615) + Rat Brain

    Predicted band size : 63 kDa
  • Anti-FAAH1 antibody (ab54615) + PC-12

    Predicted band size : 63 kDa
  • Anti-FAAH1 antibody (ab54615) + NIH/3T3

    Predicted band size : 63 kDa

References for Anti-FAAH1 antibody (ab54615)

This product has been referenced in:
  • Martin GG  et al. Female Mice are Resistant to Fabp1 Gene Ablation-Induced Alterations in Brain Endocannabinoid Levels. Lipids N/A:N/A (2016). WB ; Mouse . Read more (PubMed: 27450559) »
  • Kaczocha M  et al. Fatty acid binding protein deletion suppresses inflammatory pain through endocannabinoid/N-acylethanolamine-dependent mechanisms. Mol Pain 11:52 (2015). WB ; Mouse . Read more (PubMed: 26311517) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (A549 (mouse liver cell line), H4IIE (rat hepatoma)
Loading amount 30 µg
Specification A549 (mouse liver cell line), H4IIE (rat hepatoma
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

Submitted May 03 2013

Thank you for contacting us.

we tested catalog ab126709in IP, IHC, ICC, and Flow cytometry and it is tested negative. For this reason, we only recommending the product for western blot.

I hope this information is helpful to you. Pleas...

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Thank you for contacting us.

The ab22696 could be used at 1/10 - 1/100 dilution.
ab54615 at 5-10ug/ml
ab103724 at 5-10ug/ml
ab126709 at 1/500-1/1000 dilution.

ab6820 and ab6802 at 1/500-1/2000 dilution.

I hope thi...

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Ma collègue étant actuellement absente, c'est pourquoi je vous transmets moi-même la réponse que nous avons obtenue du laboratoire où est produit l'anticorps ab54615 :

"This antibody has been tested in F...

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Merci pour votre émail.

J'ai regardé votre protocole et le résultat. L'explication plus logique me semble qu'il existe des isoformes/modifications post-traductionnelles différentes dans le muscle et dans le cerveau. J'ai donc cherché dans la...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Neural Stem Cell)
Loading amount 20 µg
Specification Neural Stem Cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Madeleine Oudin

Verified customer

Submitted May 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Testis:seminferous tubules)
Specification Testis:seminferous tubules
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6.25
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 30 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Adult brain and lung)
Specification Adult brain and lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 08 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Parasaggital section of brain)
Specification Parasaggital section of brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"