Overview

  • Product nameAnti-FAAH2 antibody
    See all FAAH2 primary antibodies
  • Description
    Rabbit polyclonal to FAAH2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    CLGLSVYTIPSIGLALLEEKLRYSNEKYQKFKAVEESLRKELVDMLGDDG

    , corresponding to a region within C terminal amino acids 397-446 of Human FAAH2 (NP_777572)

  • Positive control
    • 721_B Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

Target

  • FunctionDegrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes monounsaturated substrate anandamide preferentially as compared to polyunsaturated substrates.
  • Tissue specificityHighly expressed in the brain, small intestine and testis. Also expressed in the heart, kidney, liver, lung and prostate.
  • Sequence similaritiesBelongs to the amidase family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMDD antibody
    • amidase domain containing antibody
    • amidase domain containing protein antibody
    • Amidase domain-containing protein antibody
    • Anandamide amidohydrolase 2 antibody
    • FAAH-2 antibody
    • FAAH2 antibody
    • FAAH2_HUMAN antibody
    • fatty acid amide hydrolase 2 antibody
    • Fatty-acid amide hydrolase 2 antibody
    • FLJ31204 antibody
    • Oleamide hydrolase 2 antibody
    • RP11-479E16 antibody
    • RP11-479E16.1 antibody
    see all

Anti-FAAH2 antibody images

  • Anti-FAAH2 antibody (ab81510) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa

References for Anti-FAAH2 antibody (ab81510)

ab81510 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HepG2, 293T, MEF)
Loading amount 20 µg
Specification HepG2, 293T, MEF
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

Submitted Jan 21 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"