Overview

  • Product name
  • Description
    Rabbit polyclonal to FAAP24
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity

    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FAAP24.

    (Peptide available as ab49605.)

  • Positive control
    • This antibody gave a positive signal in HeLa Nuclear extract, Jurkat Nuclear extract and Irradiated HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab48124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).

Target

  • Relevance
    FAAP24 was identified as a protein that interacts with FANCM as part of the core Fanconi Anemia complex. It is homologous to the XPF family of endonucleases. FAAP24 is required for normal levels of FANCD2 ubiquitination after DNA damage. Knockdown of FAAP24 leads to chromosomal instability.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • C19ORF40 antibody
    • C19orf40 chromosome 19 open reading frame 40 antibody
    • FAAP24 antibody
    • Fanconi anemia-associated protein 24 kDa antibody
    • Fanconi anemia-associated protein of 24 kDa antibody
    • FAP24_HUMAN antibody
    • MGC32020 antibody
    see all

Images

  • All lanes : Anti-FAAP24 antibody (ab48124) at 1 µg/ml

    Lane 1 : Jurkat nuclear extract lysate (ab14844)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : HeLa Whole Cell Lysate - Irradiated (5Gy

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 24 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,40 kDa. We are unsure as to the identity of these extra bands.

References

ab48124 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (various incl: HeLa, HEK293, U2OS)
Loading amount
30 µg
Specification
various incl: HeLa, HEK293, U2OS
Gel Running Conditions
Reduced Denaturing (12.5% SDS PAGE)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Dec 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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