Overview

  • Product nameAnti-FABP4 antibody
    See all FABP4 primary antibodies
  • Description
    Rabbit polyclonal to FABP4
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab13979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/200.
IP Use at an assay dependent concentration.

Target

  • FunctionLipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
  • Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • DomainForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Cellular localizationCytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3T3-L1 lipid-binding protein antibody
    • 422/aP2 antibody
    • A-FABP antibody
    • adipocyte antibody
    • Adipocyte lipid binding protein antibody
    • Adipocyte lipid-binding protein antibody
    • Adipocyte protein AP2 antibody
    • Adipocyte-type fatty acid-binding protein antibody
    • AFABP antibody
    • ALBP antibody
    • ALBP/Ap2 antibody
    • aP2 antibody
    • Epididymis secretory protein Li 104 antibody
    • FABP antibody
    • FABP4 antibody
    • FABP4_HUMAN antibody
    • Fatty acid binding protein 4 adipocyte antibody
    • Fatty acid binding protein 4 antibody
    • Fatty acid binding protein adipocyte antibody
    • Fatty acid-binding protein 4 antibody
    • Fatty acid-binding protein antibody
    • HEL S 104 antibody
    • Lbpl antibody
    • Myelin P2 protein homolog antibody
    • P15 antibody
    • P2 adipocyte protein antibody
    • Protein 422 antibody
    see all

Anti-FABP4 antibody images

  • Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.

    Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB.
  • Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.

    Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. An AlexaFluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

References for Anti-FABP4 antibody (ab13979)

This product has been referenced in:
  • Ohyama Y  et al. Differential expression of fatty acid-binding proteins and pathological implications in the progression of tongue carcinoma. Mol Clin Oncol 2:19-25 (2014). IHC-P ; Mouse . Read more (PubMed: 24649302) »
  • Nobusue H  et al. Regulation of MKL1 via actin cytoskeleton dynamics drives adipocyte differentiation. Nat Commun 5:3368 (2014). Read more (PubMed: 24569594) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us.

The antibody you have ordered is a rabbit polyclonal to FABP4 therefore, you will need to use an anti-rabbit secondary antibody so that it can recognise and bind to your primary antibody.

Unfortunately we ...

Read More

I have issued a free of charge replacement with the order number **** for the rabbit monoclonal anti-FABP4, ab92501.

To check the status of the order please contact our Customer Service team and reference this number.

Please note ...

Read More

Thank you for your call today and for letting us know about the trouble with this antibody.

Your credit note ID is ***.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on t...

Read More

Thank you for contacting Abcam regarding ab13979.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the...

Read More

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the ...

Read More

Thank you for your enquiry. The immunogen used to make this antibody was not a peptide but the full length protein. SwissProt link (http://www.uniprot.org/uniprot/P15090) I have taken the liberty BLASTing the sequence with zebrafish to check f...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas and peri-pancreatic fatty tissue)
Specification pancreas and peri-pancreatic fatty tissue
Fixative 10% NBF
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step 10% goat serum/1%BSA in TBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"