The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 µg/ml.
Use a concentration of 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
FunctionLipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Cellular localizationCytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
Adipocyte-type fatty acid-binding protein antibody
Epididymis secretory protein Li 104 antibody
Fatty acid binding protein 4 adipocyte antibody
Fatty acid binding protein 4 antibody
Fatty acid binding protein adipocyte antibody
Fatty acid-binding protein 4 antibody
Fatty acid-binding protein antibody
HEL S 104 antibody
Myelin P2 protein homolog antibody
P2 adipocyte protein antibody
Protein 422 antibody
Anti-FABP4 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody (ab23693)This image is courtesy of an anonymous Abreview
ab23693 staining FABP4 (pink) in human adipose-derived stem cells for adipogenesis by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes and blocked with 10% serum for 1 hour at 37°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Shan T et al. Lkb1 deletion promotes ectopic lipid accumulation in muscle progenitor cells and mature muscles. J Cell PhysiolN/A:N/A (2014).
Read more (PubMed: 25251157) »
Kang T et al. MicroRNA-27 (miR-27) targets prohibitin and impairs adipocyte differentiation and mitochondrial function in human adipose-derived stem cells. J Biol Chem288:34394-402 (2013).
Read more (PubMed: 24133204) »