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Anti-FABP4 antibody (ab66682)

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Overview

Product name

Anti-FABP4 antibody
See all FABP4 products (18) ...

Description

Rabbit polyclonal to FABP4

Tested applications

ICC/IF, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human FABP4.

Read Abcam's proprietary immunogen policy

(Peptide available as ab666 81.)

Positive control

This antibody gave a positive signal in the following Lysates: Mouse Skeletal Muscle Tissue Mouse Thymus Tissue Rat Ovary Tissue Rat Colon Tissue Rat Thymus Tissue HepG2 Whole Cell HeLa Whole Cell

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Fatty acids
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Stem Cells >> Mesenchymal Stem Cells >> Adipogenesis
Signal Transduction >> Metabolism >> Lipid metabolism
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Binding proteins

  • Western blot - FABP4 antibody (ab66682)Western blot - FABP4 antibody (ab66682) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab66682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 5 µg/ml.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

Target

Function

Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

Sequence similarities

Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Domain

Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

Cellular localization

Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.

Target information above from: UniProt accessionP15090 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • adipocyte antibody
  • 3T3-L1 lipid-binding protein antibody
  • 422/aP2 antibody
  • A FABP antibody
  • A-FABP antibody
  • Adipocyte lipid binding protein antibody
  • Adipocyte lipid-binding protein antibody
  • Adipocyte protein AP2 antibody
  • Adipocyte-type fatty acid-binding protein antibody
  • AFABP antibody
  • ALBP antibody
  • ALBP/Ap2 antibody
  • aP2 antibody
  • FABP antibody
  • FABP4 antibody
  • FABP4_HUMAN antibody
  • Fatty acid binding protein 4 adipocyte antibody
  • Fatty acid binding protein 4 antibody
  • Fatty acid binding protein adipocyte antibody
  • Fatty acid-binding protein 4 antibody
  • Fatty acid-binding protein antibody
  • Lbpl antibody
  • Myelin P2 protein homolog antibody
  • P15 antibody
  • P2 adipocyte protein antibody
  • Protein 422 antibody
see all

Database links

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Anti-FABP4 antibody images:

  Western blot - FABP4 antibody (ab66682)

Western blot - FABP4 antibody (ab66682)

All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml

Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
Lane 2 : Thymus (Mouse) Tissue Lysate - normal tissue (ab29285)
Lane 3 : Rat Ovary Tissue Lysate
Lane 4 : Rat Colon Tissue Lysate
Lane 5 : Rat Thymus Tissue Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 15 kDa
Observed band size : 15 kDa
Additional bands at : 18 kDa (possible post-translational modification).

References for Anti-FABP4 antibody (ab66682)

ab66682 has not yet been referenced specifically in any publications.

Publishing research using ab66682? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"