The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
Use at an assay dependent concentration. PubMed: 21489227
FunctionLipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Cellular localizationCytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
FABP4 (green) was detected using FABP4 primary antibody (ab92501; diluted 1/1000). Alpha tubulin (red) was detected using our mouse monoclonal (ab7291) antibody. Cells were imaged by confocal microscopy, using z-stack for adipocyte-like cells.
Western blot - FABP4 antibody [EPR3579] (ab92501)
All lanes : Anti-FABP4 antibody [EPR3579] (ab92501) at 1/1000 dilution
Lane 1 : Human adipose
tissue lysate Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 15 kDa Observed band size : 15 kDa
References for Anti-FABP4 antibody [EPR3579] (ab92501)
This product has been referenced in:
Fujimori K et al. Termination mechanism of CREB-dependent activation of COX-2 expression in early phase of adipogenesis. Mol Cell Endocrinol384:12-22 (2014).
Read more (PubMed: 24378735) »
Fujimori K et al. Synergistic suppression of early phase of adipogenesis by microsomal PGE synthase-1 (PTGES1)-produced PGE2 and aldo-keto reductase 1B3-produced PGF2a. PLoS One7:e44698 (2012).
Read more (PubMed: 22970288) »