The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
Use at an assay dependent concentration. PubMed: 21489227
FunctionLipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
DomainForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Cellular localizationCytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
Immunocytochemistry/Immunofluorescence analysis of 3T3-L1 (Mouse embryonic fibroblast cell line) labeling FABP4 with Purified ab92501 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
FABP4 (green) was detected using FABP4 primary antibody (ab92501; diluted 1/1000). Alpha tubulin (red) was detected using our mouse monoclonal (ab7291) antibody. Cells were imaged by confocal microscopy, using z-stack for adipocyte-like cells.
Western blot - FABP4 antibody [EPR3579] (ab92501)
All lanes : Anti-FABP4 antibody [EPR3579] (ab92501) at 1/1000 dilution
Lane 1 : Human adipose
tissue lysate Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 15 kDa Observed band size : 15 kDa
References for Anti-FABP4 antibody [EPR3579] (ab92501)
This product has been referenced in:
Li W et al. Regulation of the osteogenic and adipogenic differentiation of bone marrow-derived stromal cells by extracellular uridine triphosphate: The role of P2Y2 receptor and ERK1/2 signaling. Int J Mol Med37:63-73 (2016).
Read more (PubMed: 26531757) »
Liu Z et al. Adiponectin reduces ER stress-induced apoptosis through PPARa transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis7:e2487 (2016).
Read more (PubMed: 27882945) »