Anti-FACL4 antibody (ab110007)
Key features and details
- Goat polyclonal to FACL4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-FACL4 antibody
See all FACL4 primary antibodies -
Description
Goat polyclonal to FACL4 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide: C-
HYLKDIERMYGGK
, corresponding to C terminal amino acids 699-711 of Human FACL4 -
Positive control
- Human brain (cortex) tissue, Human small intestine tissue, HepG2 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 79 kDa.
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IHC-P |
Use a concentration of 3.75 µg/ml.
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Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 79 kDa. |
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
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Function
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates. -
Involvement in disease
Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis. -
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family. -
Cellular localization
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 536628 Cow
- Entrez Gene: 481018 Dog
- Entrez Gene: 2182 Human
- Entrez Gene: 50790 Mouse
- Entrez Gene: 448980 Pig
- Entrez Gene: 113976 Rat
- Omim: 300157 Human
- SwissProt: O60488 Human
see all -
Alternative names
- ACS 4 antibody
- ACS4 antibody
- ACSL 4 antibody
see all
Images
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ab110007, at 3.75 µg/ml, staining FACL4 in human brain (cortex) tissue by immunohistochemistry.
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ab110007, at 3.75 µg/ml, staining FACL4 in Human small intestine tissue by immunohistochemistry.
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Anti-FACL4 antibody (ab110007) at 0.1 µg/ml + HepG2 cell lysate in RIPA buffer at 35 µg
Predicted band size: 79 kDa
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab110007 has been referenced in 5 publications.
- Zhang L et al. S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis. Cell Mol Gastroenterol Hepatol 11:697-724 (2021). PubMed: 33075563
- Fan Y et al. Identification of Milk Fat Metabolism-Related Pathways of the Bovine Mammary Gland during Mid and Late Lactation and Functional Verification of the ACSL4 Gene. Genes (Basel) 11:N/A (2020). PubMed: 33207796
- Wang J et al. The positive feedback between ACSL4 expression and O-GlcNAcylation contributes to the growth and survival of hepatocellular carcinoma. Aging (Albany NY) 12:7786-7800 (2020). PubMed: 32357142
- Sun XJ & Xu GL Overexpression of Acyl-CoA Ligase 4 (ACSL4) in Patients with Hepatocellular Carcinoma and its Prognosis. Med Sci Monit 23:4343-4350 (2017). PubMed: 28887439
- Lynes EM et al. Palmitoylation is the switch that assigns calnexin to quality control or ER Ca2+ signaling. J Cell Sci 126:3893-903 (2013). PubMed: 23843619