Anti-FACL4 antibody - Aminoterminal end (ab70937)

Overview

  • Product name
    Anti-FACL4 antibody - Aminoterminal end
    See all FACL4 primary antibodies
  • Description
    Rabbit polyclonal to FACL4 - Aminoterminal end
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the N-terminal region of human FACL4.

  • Positive control
    • Human hepatocarcinoma tissue and 293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab70937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notes
    ELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/100 - 1/500. Detects bands of approximately 28, 30 and 65 kDa (predicted molecular weight: 79 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
    • Involvement in disease
      Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
      Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis.
    • Sequence similarities
      Belongs to the ATP-dependent AMP-binding enzyme family.
    • Cellular localization
      Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACS 4 antibody
      • ACS4 antibody
      • ACSL 4 antibody
      • Acsl4 antibody
      • ACSL4_HUMAN antibody
      • acyl CoA synthetase 4 antibody
      • Acyl CoA synthetase long chain family member 4 antibody
      • FACL 4 antibody
      • FACL4 antibody
      • Fatty acid Coenzyme A ligase antibody
      • fatty acid Coenzyme A ligase long-chain 4 antibody
      • LACS 4 antibody
      • LACS4 antibody
      • Lignoceroyl CoA synthase antibody
      • Long chain 4 antibody
      • long chain acyl CoA synthetase 4 antibody
      • long chain fatty acid CoA ligase 4 antibody
      • long chain fatty acid Coenzyme A ligase 4 antibody
      • Long-chain acyl-CoA synthetase 4 antibody
      • Long-chain-fatty-acid--CoA ligase 4 antibody
      • MRX63 antibody
      • MRX68 antibody
      see all

    Images

    • Anti-FACL4 antibody - Aminoterminal end (ab70937) at 1/100 dilution + 293 cell lysate at 12.5 µg

      Predicted band size : 79 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 28 kDa,30 kDa. We are unsure as to the identity of these extra bands.
    • Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with ab70937 at a 1:50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

    References

    ab70937 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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