Recombinant Anti-FACL4 antibody [EPR8640] (ab155282)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8640] to FACL4
- Suitable for: Flow Cyt (Intra), IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FACL4 antibody [EPR8640]
See all FACL4 primary antibodies -
Description
Rabbit monoclonal [EPR8640] to FACL4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human FACL4. The exact sequence is proprietary.
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Positive control
- WB: HepG2, HeLa and 293T cell lysates and human fetal kidney tissue, mouse brain tissue, rat brain, kidney and spleen tissue lysates. IHC-P: human hepatocellular carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt (intra): Permeabilized 293T cells. IP: HepG2 whole cell lysate (ab7900).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8640 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Hep G2 whole cell lysate (ab166833)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/10 - 1/100.
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WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 79 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
WB
1/10000 - 1/50000. Predicted molecular weight: 79 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates. -
Involvement in disease
Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis. -
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family. -
Cellular localization
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2182 Human
- Entrez Gene: 50790 Mouse
- Entrez Gene: 113976 Rat
- Omim: 300157 Human
- SwissProt: O60488 Human
- SwissProt: Q9QUJ7 Mouse
- SwissProt: O35547 Rat
- Unigene: 268785 Human
see all -
Alternative names
- ACS 4 antibody
- ACS4 antibody
- ACSL 4 antibody
see all
Images
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All lanes : Anti-FACL4 antibody [EPR8640] (ab155282) at 1/10000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Anti-FACL4 antibody [EPR8640] (ab155282) at 1/50000 dilution (purified) + HepG2 whole cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-FACL4 antibody [EPR8640] (ab155282) at 1/10000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293 whole cell lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugate goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FACL4 with purified ab155282 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FACL4 with purified ab155282 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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Intracellular Flow Cytometry analysis of 293T cells labelling FACL4 with purified ab155282 at 1/100 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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ab155282 (purified) at 1/30 immunoprecipitating FACL4 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10µg)
Lane 2 (+): ab155282 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155282 in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-FACL4 antibody [EPR8640] (ab155282) at 1/10000 dilution (unpurified)
Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : 293T lysate
Lane 4 : Fetal kidney lysate, human
Lysates/proteins at 10 µg per lane.
Predicted band size: 79 kDa -
All lanes : Anti-FACL4 antibody [EPR8640] (ab155282) at 1/10000 dilution (unpurified)
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : Rat spleen tissue lysate
Predicted band size: 79 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
Intracellular flow cytometric analysis of permeabilized 293T cells labelling FACL4with unpurified ab155282 at a dilution of 1/10 (red) compared to a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (79)
ab155282 has been referenced in 79 publications.
- Liu Q et al. PUM2 aggravates the neuroinflammation and brain damage induced by ischemia-reperfusion through the SLC7A11-dependent inhibition of ferroptosis via suppressing the SIRT1. Mol Cell Biochem 478:609-620 (2023). PubMed: 35997855
- Li B et al. Knockdown of SHP2 attenuated LPS-induced ferroptosis via downregulating ACSL4 expression in acute lung injury. Allergol Immunopathol (Madr) 51:143-152 (2023). WB . PubMed: 37169572
- Zhang B et al. LncRNA HEPFAL accelerates ferroptosis in hepatocellular carcinoma by regulating SLC7A11 ubiquitination. Cell Death Dis 13:734 (2022). PubMed: 36008384
- Li M et al. Melatonin Inhibits the Ferroptosis Pathway in Rat Bone Marrow Mesenchymal Stem Cells by Activating the PI3K/AKT/mTOR Signaling Axis to Attenuate Steroid-Induced Osteoporosis. Oxid Med Cell Longev 2022:8223737 (2022). PubMed: 36035224
- Zhang Y et al. MITD1 Deficiency Suppresses Clear Cell Renal Cell Carcinoma Growth and Migration by Inducing Ferroptosis through the TAZ/SLC7A11 Pathway. Oxid Med Cell Longev 2022:7560569 (2022). PubMed: 36046690