Overview

  • Product name
    FACS Blue LacZ beta Galactosidase detection kit
    See all beta galactosidase kits
  • Product overview

    Although chromogenic assays of β-galactosidase activity (i.e. X-Gal) have use, application of the fluorogenic substrate 3-carboxyumbelliferyI β-D-galactopyranoside (CUG) (blue fluorescence) combined with Fluorescence Activated Cell Sorting (FACS) analysis has been shown to be several orders of magnitude more sensitive. In addition, because of its high water solubility and detection limits, the CUG substrate has found extensive use in automated ELISA type assay systems. Abcam’s FACS Blue LacZ beta Galactosidase detection kit (ab189815) is especially useful for dual-labeling experiments where the cells to be analyzed are also labeled with a fluorescein based probe (FITC-labeled antibody, fluorescein-based substrate, etc.).

    Emission: 460 nm.

    Excitation: 390 nm.

  • Notes

    One of the most common reporter genes used in molecular biology applications is the E. coli lacZ gene that codes for an active subunit of β-galactosidase in vivo. Since this enzyme is generally absent in normal mammalian, yeast, some bacterial and even plant cells, it can be detected at very low levels, and since the enzyme has a wide substrate specificity, monitoring lacZ expression (and therefore co-expressed genes or promoter efficiency) has become routine to the point of detection of as few as 5 copies of β-galactosidase per cell by FACS analysis.

    Abcam’s FACS Blue LacZ beta Galactosidase detection kit (ab189815) provides reagents and protocol to perform up to 500 tests (10 x 96 well microtitre plates).

  • Tested applications
    Suitable for: Othermore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 500 tests
    Inhibitor 1 x 1ml
    Reference Standard 1 x 0.5ml
    Substrate Reagent 1 x 0.5ml
  • Research areas
  • Relevance
    Beta galactosidase is a hydrolase enzyme that cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Beta galactosidase is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. Senescent cells display senescence-associated expression of beta galactosidase activity.
  • Cellular localization
    Isoform 1: Lysosome. Isoform 2: Cytoplasm, perinuclear region. Note=Localized to the perinuclear area of the cytoplasm but not to lysosomes.
  • Alternative names
    • Acid beta galactosidase
    • Beta galactosidase 1
    • EBP
    • ELNR1
    • Galactosidase beta 1
    • GLB1
    • MPS4B
    see all

Applications

Our Abpromise guarantee covers the use of ab189815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Other Use at an assay dependent concentration.

Measures the expression of reporter gene, beta-galactosidase.

Protocols

References

This product has been referenced in:
  • Timraz SB  et al. In-depth evaluation of commercially available human vascular smooth muscle cells phenotype: Implications for vascular tissue engineering. Exp Cell Res 343:168-76 (2016). Read more (PubMed: 27079869) »

See 1 Publication for this product

Customer reviews and Q&As



In regards to your question on reagentand preparation, the substrate reagent issupped at 50 mM (and comes as distilled water, for your information only no adding of water is necessary), you add 0.5 ml of the reagent to 2 ml of reaction buffe...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up