The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250. as capture antibody. The dilution guideline for ELISA is based on sandwich ELISA in combination with a polyclonal antibody against the antigen.
Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 27 kDa.
FunctionFactor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Involvement in diseaseDefects in CFD are the cause of complement factor D deficiency (CFD deficiency) [MIM:134350]. CFD deficiency predisposes to invasive meningococcal disease.
Sequence similaritiesBelongs to the peptidase S1 family. Contains 1 peptidase S1 domain.