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Human 155 kDa Factor H purified from plasma.
Factor H is mainly synthesised in the liver but also in macrophages and endothelium. It is primarily aplasma glycoprotein but is also found in platelets and there is a membrane bound form on some leukocytes. Consisting of a single polypeptide, the major form of Factor H has a molecular weight of 155kDa. There are two truncated forms, a non-glycosylated 49 kDa form and a glycosylated 39-43 kDaform. Plasma concentrations are in the range 200-600mg/L for the 155 kDa form and 1-5mg/L for thetruncated forms. Factor H is a major regulatory protein of the complement system. By binding to C3b it either displacesor prevents the binding of Bb (activated Factor B). When bound to Factor H, C3b is susceptible tocleavage by Factor 1 to yield iC3b. Factor H is released or modified following this cleavage. The regulatory role of Factor H is essential because C3bBb is not only a C5 convertase but a C3 convertaseand so has a positive feedback effect, potentially consuming the entire C3 pool if unregulated.
Our Abpromise guarantee covers the use of ab8842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. PubMed: 20813971
Suitability for use in nephelometry and enzyme-linked immunosorbent assays have not been assessed but use in such assays should not necessarily be excluded.
|IHC-P||Use at an assay dependent concentration. PubMed: 20813971|
|Flow Cyt||Use at an assay dependent concentration. ab37385-Sheep polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|Double Immunodiffusion||Use at an assay dependent concentration. 10µL antiserum vs 10µL plasma|
|RID||Use at an assay dependent concentration. (and Rocket IEP) 1µL antiserum/cm² gel vs 5µL neat - 1/10 dilution human plasma.
The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
|Immunoelectrophoresis||Use at an assay dependent concentration. 100µL antiserum vs 5µL plasma|
|Other||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration. PubMed: 22461909|
Image from Bhide MR et al, BMC Res Notes. 2009 Jul 15;2:134, Fig 1.