Overview

  • Product nameAnti-Factor IX antibody [13B9]
    See all Factor IX primary antibodies
  • Description
    Mouse monoclonal [13B9] to Factor IX
  • SpecificityThis antibody has specificity for native human factor IX.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) isolated from human plasma.

  • EpitopeEpitope specificity differs from that of ab17196 as determined by inhibition ELISA.

Properties

Applications

Our Abpromise guarantee covers the use of ab17197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/12500.
WB Use at an assay dependent dilution. Predicted molecular weight: 51 kDa. The antibody reacts with factor IX in a reduced as well as in a non-reduced form.

Target

  • FunctionFactor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
  • Tissue specificitySynthesized primarily in the liver and secreted in plasma.
  • Involvement in diseaseDefects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
    Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
    Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • DomainCalcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain.
  • Post-translational
    modifications
    Activated by factor XIa, which excises the activation peptide.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Christmas Disease antibody
    • Christmas factor antibody
    • Coagulant factor IX antibody
    • Coagulation factor 9 antibody
    • Coagulation factor IX antibody
    • Coagulation factor IXa heavy chain antibody
    • F9 antibody
    • FA9_HUMAN antibody
    • Factor 9 antibody
    • Factor IX Deficiency antibody
    • FactorIX antibody
    • FIX antibody
    • Haemophilia B antibody
    • HEMB antibody
    • MGC129641 antibody
    • MGC129642 antibody
    • P19 antibody
    • Plasma Thromboplastic Component antibody
    • Plasma thromboplastin component antibody
    • PTC antibody
    see all

Anti-Factor IX antibody [13B9] images

  • All lanes : Anti-Factor IX antibody [13B9] (ab17197) at 5 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Factor IX antibody [13B9] (ab17197)

ab17197 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"