Overview

  • Product nameAnti-Factor Va antibody
  • Description
    Sheep polyclonal to Factor Va
  • SpecificityThis antibody recognizes Human Factor Va.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Native, purified human Factor Va

  • General notesConcentration 5-10mg/ml (lot specific).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol/ Water
  • Concentration information loading...
  • PurityIon Exchange Chromatography
  • Purification notesSalt fractionation followed by ion exchange chromatography on DEAE sepharose.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 168 kDa.

Target

  • RelevanceFactor Va is a cofactor for the serine protease factor Xa, and in the presence of calcium ions they collectively assemble on a phospholipid surface to form the prothrombinase complex. The prothrombinase complex is responsible for the rapid conversion of prothrombin to thrombin. Factor Va is derived from the pro-cofactor, factor V, upon limited proteolysis by alpha-thrombin. The thrombin cleavage of factor V liberates two heavily glycosylated activation peptides from the central portion of the molecule which have no cofactor function. Factor Va is comprised of an NH2-terminal derived heavy chain (MW 94kDa) and a COOH-terminal derived light chain (MW 74kDa) which remain associated in the presence of calcium ions. The cofactor binds to phospholipid (cell membrane) surfaces and effectively serves as a receptor for membrane bound factor Xa. Complete assembly of the prothrombinase complex (factor Xa, factor Va, phospholipid, and calcium) results in a 300,000-fold increase in the rate of prothrombin conversion relative to the rate observed with factor Xa alone. The interaction between factor Va and factor Xa is mediated by both the heavy and light chain of factor Va, while the binding of prothrombin to factor Va is mediated solely by the heavy chain.
  • Cellular localizationCell Membrane and Plasma membrane
  • Database links
  • Alternative names
    • Activated protein c cofactor antibody
    • Coagulation factor V (proaccelerin, labile factor) antibody
    • Coagulation factor V antibody
    • F5 antibody
    • Factor V Leiden antibody
    • FVL antibody
    • PCCF antibody
    see all

References for Anti-Factor Va antibody (ab81460)

ab81460 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"