Overview

  • Product nameAnti-Factor VII antibody
    See all Factor VII primary antibodies
  • Description
    Rabbit polyclonal to Factor VII
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 209 and 444 of Human Factor VII.

  • Positive control
    • WB: NIH 3T3, H1299, 293T, A431, HeLaS3, HepG2, MOLT4 or Raji cell lysate; IHC-P: gastric carcinoma tissue. IF/ICC: HepG2 cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/500.

Target

  • FunctionInitiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. FA7D is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • coagulation factor VII (serum prothrombin conversion accelerator) antibody
    • Coagulation factor VII antibody
    • Eptacog alfa antibody
    • F7 antibody
    • FA7_HUMAN antibody
    • Factor VII antibody
    • Factor VII heavy chain antibody
    • Factor VII light chain antibody
    • FVII coagulation protein antibody
    • OTTHUMP00000018733 antibody
    • OTTHUMP00000018734 antibody
    • Proconvertin antibody
    • Serum prothrombin conversion accelerator antibody
    • SPCA antibody
    see all

Anti-Factor VII antibody images

  • ICC/IF image of ab97614 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97614, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-Factor VII antibody (ab97614) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 52 kDa
    10% SDS PAGE
  • Anti-Factor VII antibody (ab97614) at 1/500 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 52 kDa
    7.5% SDS PAGE
  • Immunohistochemistry analysis of paraffin-embedded N human (gastric carcinoma) tissue, using ab97614 at 1/100 dilution.

References for Anti-Factor VII antibody (ab97614)

This product has been referenced in:
  • Chen KD  et al. Interconnections between autophagy and the coagulation cascade in hepatocellular carcinoma. Cell Death Dis 5:e1244 (2014). WB ; Human . Read more (PubMed: 24853422) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab97614.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"