Overview

  • Product name
  • Description
    Rabbit polyclonal to Factor VIIa
  • Tested applications
    Suitable for: Prothrombin Assay, Indirect ELISA, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein (Human) (NovoSeven®).

  • General notes
    0.02% sodium azide can be added as a preservative if storing at 4°C

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Prothrombin Assay
Indirect ELISA
Inhibition Assay
  • Application notes
    I-ELISA: 1/15000 for antibodies at 0.1 mg/ml to generate absorbance 1.0 at 450 nm after 10 minutes of incubation with OPD tablets at room temperature.
    Inhib: Use at an assay dependant dilution.
    PA: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
    • Tissue specificity
      Plasma.
    • Involvement in disease
      Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. A hemorrhagic disease with variable presentation. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or repeated hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Finally, numerous subjects are completely asymptomatic despite very low factor VII levels.
    • Sequence similarities
      Belongs to the peptidase S1 family.
      Contains 2 EGF-like domains.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 1 peptidase S1 domain.
    • Post-translational
      modifications
      The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
      O- and N-glycosylated. N-glycosylation at Asn-205 occurs cotranslationally and is mediated by STT3A-containing complexes, while glycosylation at Asn-382 is post-translational and is mediated STT3B-containing complexes before folding. O-fucosylated by POFUT1 on a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Coagulation factor VII antibody
      • Eptacog alfa antibody
      • F7 antibody
      • FA7_HUMAN antibody
      • Factor VII heavy chain antibody
      • FVII coagulation protein antibody
      • Proconvertin antibody
      • Serum prothrombin conversion accelerator antibody
      • SPCA antibody
      see all

    References for Anti-Factor VIIa antibody (ab7053)

    This product has been referenced in:
    • Kamocka M  et al. Rat prostate tumors express cancer procoagulant, an activator of coagulation factor X. Comp Med 58:282-6 (2008). ELISA . Read more (PubMed: 18589871) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. For ab21037, the antibody was tested against purified Human Factor V and against human plasma in ELISA and Western blot. The characterization of ab20951, MAb to Factor V (Clone 701), was described in the following publish...

    Read More

    Thank you for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

    I'm afraid that we are unable to answer your customer's question. We know that this antibody cross reacts with Factor VII, but do not have any further information about the relative degree of cross reactivity.

    No, this is our only antibody against factor VIIa

    The only other species tested has been rat.

    This antibody has not beed tested on the Western Blot. However, it binds very strongly to native Factor VIIa and Factor VII. No, the cross-reactivity has not been determined. Technical Support- AgriSera

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up