Overview

  • Product name
    Anti-Factor VIII antibody [27.4]
    See all Factor VIII primary antibodies
  • Description
    Mouse monoclonal [27.4] to Factor VIII
  • Specificity
    ab41188 does not cross react with the von Willebrand factor.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    N-terminal region of the 83kD light chain of purified human Factor VIII.

  • Epitope
    ab41188 recognises an epitope in the N-terminal region of the 83kD light chain of Factor VIII.
  • General notes
    Stable for at least 1 year at -20°C to -70°C.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    27.4
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/16,000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 267 kDa.

    Dilute in PBS or medium which is identical to that used in the assay system.

    Can inhibit Factor VIII activity in coagulation assays.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
    • Involvement in disease
      Defects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
    • Sequence similarities
      Belongs to the multicopper oxidase family.
      Contains 3 F5/8 type A domains.
      Contains 2 F5/8 type C domains.
      Contains 6 plastocyanin-like domains.
    • Domain
      Domain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
    • Post-translational
      modifications
      Sulfation on Tyr-1699 is essential for binding vWF.
    • Cellular localization
      Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • AHF antibody
      • Antihemophilic factor antibody
      • Coagulation factor VIII antibody
      • coagulation factor VIII, procoagulant component antibody
      • coagulation factor VIIIc antibody
      • DXS1253E antibody
      • F8 antibody
      • F8b antibody
      • F8c antibody
      • FA8_HUMAN antibody
      • factor VIII F8B antibody
      • Factor VIIIa light chain antibody
      • FactorVIII antibody
      • FVIII antibody
      • Hema antibody
      • Hemophilia A antibody
      • Hemophilia, classic antibody
      • OTTHUMP00000061446 antibody
      • OTTHUMP00000196174 antibody
      • Procoagulant component antibody
      see all

    References for Anti-Factor VIII antibody [27.4] (ab41188)

    This product has been referenced in:
    • Pandey GS  et al. Detection of intracellular Factor VIII protein in peripheral blood mononuclear cells by flow cytometry. Biomed Res Int 2013:793502 (2013). Read more (PubMed: 23555096) »

    See 1 Publication for this product

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