Anti-Factor VIII antibody [27.4] (ab41188)


  • Product nameAnti-Factor VIII antibody [27.4]
    See all Factor VIII primary antibodies
  • Description
    Mouse monoclonal [27.4] to Factor VIII
  • Specificityab41188 does not cross react with the von Willebrand factor.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    N-terminal region of the 83kD light chain of purified human Factor VIII.

  • Epitopeab41188 recognises an epitope in the N-terminal region of the 83kD light chain of Factor VIII.
  • General notesStable for at least 1 year at -20°C to -70°C.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number27.4
  • IsotypeIgG2a
  • Research areas


Our Abpromise guarantee covers the use of ab41188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/16,000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 267 kDa.

    Dilute in PBS or medium which is identical to that used in the assay system.

    Can inhibit Factor VIII activity in coagulation assays.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFactor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
    • Involvement in diseaseDefects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
    • Sequence similaritiesBelongs to the multicopper oxidase family.
      Contains 3 F5/8 type A domains.
      Contains 2 F5/8 type C domains.
      Contains 6 plastocyanin-like domains.
    • DomainDomain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
    • Post-translational
      Sulfation on Tyr-1699 is essential for binding vWF.
    • Cellular localizationSecreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • AHF antibody
      • Antihemophilic factor antibody
      • Coagulation factor VIII antibody
      • coagulation factor VIII, procoagulant component antibody
      • coagulation factor VIIIc antibody
      • DXS1253E antibody
      • F8 antibody
      • F8b antibody
      • F8c antibody
      • FA8_HUMAN antibody
      • factor VIII F8B antibody
      • Factor VIIIa light chain antibody
      • FactorVIII antibody
      • FVIII antibody
      • Hema antibody
      • Hemophilia A antibody
      • Hemophilia, classic antibody
      • OTTHUMP00000061446 antibody
      • OTTHUMP00000196174 antibody
      • Procoagulant component antibody
      see all

    References for Anti-Factor VIII antibody [27.4] (ab41188)

    ab41188 has not yet been referenced specifically in any publications.

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