• Product nameAnti-Factor VIII antibody
    See all Factor VIII primary antibodies
  • Description
    Rat monoclonal to Factor VIII
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length Mouse Factor VIII


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Water
  • Concentration information loading...
  • PurityIon Exchange Chromatography
  • Purification notesSalt fractionation followed by gel filtration and ion exchange chromatography
  • ClonalityMonoclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab61390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 267 kDa.
ELISA Use at an assay dependent dilution.


  • FunctionFactor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
  • Involvement in diseaseDefects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
  • Sequence similaritiesBelongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 2 F5/8 type C domains.
    Contains 6 plastocyanin-like domains.
  • DomainDomain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
  • Post-translational
    Sulfation on Tyr-1699 is essential for binding vWF.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHF antibody
    • Antihemophilic factor antibody
    • Coagulation factor VIII antibody
    • coagulation factor VIII, procoagulant component antibody
    • coagulation factor VIIIc antibody
    • DXS1253E antibody
    • F8 antibody
    • F8b antibody
    • F8c antibody
    • FA8_HUMAN antibody
    • factor VIII F8B antibody
    • Factor VIIIa light chain antibody
    • FactorVIII antibody
    • FVIII antibody
    • Hema antibody
    • Hemophilia A antibody
    • Hemophilia, classic antibody
    • OTTHUMP00000061446 antibody
    • OTTHUMP00000196174 antibody
    • Procoagulant component antibody
    see all

References for Anti-Factor VIII antibody (ab61390)

This product has been referenced in:
  • Follenzi A  et al. Role of bone marrow transplantation for correcting hemophilia A in mice. Blood 119:5532-42 (2012). ICC/IF ; Mouse . Read more (PubMed: 22368271) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (2963T cell that was lenti viral transduced with B)
Specification 2963T cell that was lenti viral transduced with B
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 11 2013

Thank you for contacting Abcam.

We have not determined the epitope for ab61390 and so I am unable to provide any more information. As for ab53703, it appears to have been removed from the catalogue due to licensing issues and not because of a...

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Thank you for your further enquiry regarding ab61390.   I can confirm that ab61390 has been tested for Western blot application but currently we do not have image available for the datasheet yet. Western blotting was performed against recombinant ...

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