Anti-Factor VIII antibody [GMA-012] (ab78852)

Overview

  • Product nameAnti-Factor VIII antibody [GMA-012]
    See all Factor VIII primary antibodies
  • Description
    Mouse monoclonal [GMA-012] to Factor VIII
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human Factor VIII

  • Positive control
    • Human Factor VIII. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1% Mannitol, 0.15M Sodium chloride, 10mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • PurityDEAE-Chromatography
  • ClonalityMonoclonal
  • Clone numberGMA-012
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 280 kDa (predicted molecular weight: 267 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionFactor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
  • Involvement in diseaseDefects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
  • Sequence similaritiesBelongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 2 F5/8 type C domains.
    Contains 6 plastocyanin-like domains.
  • DomainDomain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
  • Post-translational
    modifications
    Sulfation on Tyr-1699 is essential for binding vWF.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHF antibody
    • Antihemophilic factor antibody
    • Coagulation factor VIII antibody
    • coagulation factor VIII, procoagulant component antibody
    • coagulation factor VIIIc antibody
    • DXS1253E antibody
    • F8 antibody
    • F8b antibody
    • F8c antibody
    • FA8_HUMAN antibody
    • factor VIII F8B antibody
    • Factor VIIIa light chain antibody
    • FactorVIII antibody
    • FVIII antibody
    • Hema antibody
    • Hemophilia A antibody
    • Hemophilia, classic antibody
    • OTTHUMP00000061446 antibody
    • OTTHUMP00000196174 antibody
    • Procoagulant component antibody
    see all

Anti-Factor VIII antibody [GMA-012] images

  • Anti-Factor VIII antibody [GMA-012] (ab78852) at 1 µg/ml + human Factor VIII at 2 µg

    Secondary
    Goat anti-mouse HRP conjugated IgG

    Predicted band size : 267 kDa
    Observed band size : 280 kDa (why is the actual band size different from the predicted?)
  • IHC image of Factor VIII staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78852, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Factor VIII antibody [GMA-012] (ab78852)

ab78852 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"