Anti-Factor VIII + VIII C2domain antibody (ab168752)
Key features and details
- Rabbit polyclonal to Factor VIII + VIII C2domain
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Factor VIII + VIII C2domain antibody -
Description
Rabbit polyclonal to Factor VIII + VIII C2domain -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Dog -
Immunogen
Recombinant full length protein within Human Factor VIII + VIII C2domain aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Factor VIII + VIII C2domain transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. |
Target
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Relevance
Haemophilia A is an X-linked, recessive, bleeding disorder caused by a deficiency in the activity of coagulation factor VIII. Affected individuals develop a variable phenotype of haemorrhage into joints and muscles, easy bruising, and prolonged bleeding from wounds. The disorder is caused by heterogeneous mutations in the factor VIII gene. Up to 30% of haemophilia A patients given therapeutic fVIII can make inhibitory antibodies, the majority of which are reactive with its C2 and A2 domains. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 403875 Dog
- Entrez Gene: 2157 Human
- Entrez Gene: 14069 Mouse
- Omim: 306700 Human
- SwissProt: O18806 Dog
- SwissProt: P00451 Human
- SwissProt: Q06194 Mouse
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Alternative names
- AHF antibody
- Antihemophilic factor antibody
- Coagulation factor VIII antibody
see all
Images
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All lanes : Anti-Factor VIII + VIII C2domain antibody (ab168752) at 1 µg/ml
Lane 1 : Factor VIII + VIII C2domain transfected 293T cell lysate (24.60 KDa)
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated secondary antibody at 1/7500 dilution
Predicted band size: 27 kDa
Datasheets and documents
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Datasheet download
References (0)
ab168752 has not yet been referenced specifically in any publications.