Overview

  • Product nameAnti-Factor X antibody
    See all Factor X primary antibodies
  • Description
    Mouse monoclonal to Factor X
  • SpecificityThis antibody recognizes the heavy chains of human factors Xa and X; it does not bind bovine factor X or BEGRck-Xa.
  • Tested applicationsSuitable for: WB, ELISA, Purificationmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native purified Human Factor X

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% (v/v)Glycerol/water
  • Concentration information loading...
  • PurityIon Exchange Chromatography
  • Purification notesSalt fractionation followed by gel filtration and ion exchange chromatography.
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 55 kDa.
ELISA Use at an assay dependent dilution.
Purification Use at an assay dependent dilution. This antibody inhibits PT, prothrombinase and partially APTT, but not amidase activity.

Target

  • FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • Tissue specificityPlasma; synthesized in the liver.
  • Involvement in diseaseDefects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • FormCleaved into the following 3 chains: 1. Factor X light chain 2. Factor X heavy chain 3. Activated factor Xa heavy chain
  • Alternative names
    • Activated factor Xa heavy chain antibody
    • Coagulation factor X antibody
    • F10 antibody
    • FA10_HUMAN antibody
    • factor Xa antibody
    • FX antibody
    • FXA antibody
    • Prothrombinase antibody
    • Stuart factor antibody
    • Stuart Prower factor antibody
    • Stuart-Prower factor antibody
    see all

References for Anti-Factor X antibody (ab61361)

This product has been referenced in:
  • Böhm A  et al. Factor-Xa-induced mitogenesis and migration require sphingosine kinase activity and S1P formation in human vascular smooth muscle cells. Cardiovasc Res 99:505-13 (2013). WB, IHC-P ; Human . Read more (PubMed: 23658376) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"