Overview

  • Product nameAnti-Factor X antibody
    See all Factor X primary antibodies
  • Description
    Rat monoclonal to Factor X
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Full length native purified Mouse Factor X

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% (v/v)Glycerol/water
  • Concentration information loading...
  • PurityIon Exchange Chromatography
  • Purification notesSalt fractionation followed by gel filtration and ion exchange chromatography.
  • ClonalityMonoclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 55 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • Tissue specificityPlasma; synthesized in the liver.
  • Involvement in diseaseDefects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • FormCleaved into the following 3 chains: 1. Factor X light chain 2. Factor X heavy chain 3. Activated factor Xa heavy chain
  • Alternative names
    • Activated factor Xa heavy chain antibody
    • Coagulation factor X antibody
    • F10 antibody
    • FA10_HUMAN antibody
    • factor Xa antibody
    • FX antibody
    • FXA antibody
    • Prothrombinase antibody
    • Stuart factor antibody
    • Stuart Prower factor antibody
    • Stuart-Prower factor antibody
    see all

References for Anti-Factor X antibody (ab61389)

ab61389 has not yet been referenced specifically in any publications.

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Smearing could come from too much albumin and IgGs in the sample (these are inherent to plasma samples and dominate over other proteins in the plasma). Thus, removing those beforehand could help as well. There are columns commercially available specifi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"