Overview

  • Product nameAnti-Factor X antibody
    See all Factor X primary antibodies
  • Description
    Rabbit polyclonal to Factor X
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, WB, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human Factor X purified from human plasma

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.5
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Flow Cyt
IHC-P
WB
RIA
  • Application notes EIA/RIA: Use at a concentration of 5 µg/ml.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 55 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
    • Tissue specificityPlasma; synthesized in the liver.
    • Involvement in diseaseDefects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
    • Sequence similaritiesBelongs to the peptidase S1 family.
      Contains 2 EGF-like domains.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 1 peptidase S1 domain.
    • Post-translational
      modifications
      The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
      N- and O-glycosylated.
      The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • FormCleaved into the following 3 chains: 1. Factor X light chain 2. Factor X heavy chain 3. Activated factor Xa heavy chain
    • Alternative names
      • Activated factor Xa heavy chain antibody
      • Coagulation factor X antibody
      • F10 antibody
      • FA10_HUMAN antibody
      • factor Xa antibody
      • FX antibody
      • FXA antibody
      • Prothrombinase antibody
      • Stuart factor antibody
      • Stuart Prower factor antibody
      • Stuart-Prower factor antibody
      see all

    Anti-Factor X antibody images

    • ICC/IF image of ab79929 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79929 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • IHC image of Factor X staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79929, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References for Anti-Factor X antibody (ab79929)

    ab79929 has not yet been referenced specifically in any publications.

    Product Wall

    Application Flow Cytometry
    Fixation Formaldehyde
    Sample Human Cell (hepatocyte)
    Specification hepatocyte
    Permeabilization Yes - 0.1% triton
    Username

    Dr. Xaowen He

    Verified customer

    Submitted Nov 27 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"