• Product nameAnti-Factor X antibody
    See all Factor X primary antibodies
  • Description
    Rabbit polyclonal to Factor X
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: IVSGFGRTHE KGRQSTRLKM LEVPYVDRNS CKLSSSFIIT QNMFCAGYDT, corresponding to amino acids 360-409 of Human Factor X (NP_000495).

  • Positive control
    • 721_B cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab80345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.


  • FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • Tissue specificityPlasma; synthesized in the liver.
  • Involvement in diseaseDefects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • FormCleaved into the following 3 chains: 1. Factor X light chain 2. Factor X heavy chain 3. Activated factor Xa heavy chain
  • Alternative names
    • Activated factor Xa heavy chain antibody
    • Coagulation factor X antibody
    • F10 antibody
    • FA10_HUMAN antibody
    • factor Xa antibody
    • FX antibody
    • FXA antibody
    • Prothrombinase antibody
    • Stuart factor antibody
    • Stuart Prower factor antibody
    • Stuart-Prower factor antibody
    see all

Anti-Factor X antibody images

  • Anti-Factor X antibody (ab80345) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)

References for Anti-Factor X antibody (ab80345)

ab80345 has not yet been referenced specifically in any publications.

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