Factor X Human Chromogenic Activity Assay Kit (ab108833)

Overview

  • Product nameFactor X Human Chromogenic Activity Assay Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Plasma
  • Assay typeQuantitative
  • Sensitivity
    = 0.125 µg/ml
  • Range
    0.125 µg/ml - 8 µg/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    Factor X Human Chromogenic Activity Assay Kit (ab108833) is developed to determine human Factor X activity in plasma and cell culture. The assay measures the activation of zymogen Factor X to Factor Xa by RVV. The amidolytic activity of the Factor Xa is quantitated using a highly specific Factor Xa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the Factor X enzymatic activity.

     

    High level of FX activity samples: range of 0.125-8 µg/ml Low level of FX activity samples: range of 0.0156-1 µg/ml

  • Notes

    Factor X is a plasma serine protease zymogen involved in the blood coagulation cascade. Factor X is purified from plasma as a two-chain protein consisting of a 45-kDa heavy chain and a 17-kDa light chain. The Factor X heavy chain is cleaved during coagulation by several different proteases including the intrinsic Xase complex, the Factor X-activating enzyme from Russell’s viper venom (RVV) and trypsin, and also by extrinsic (tissue Factor/Factor VIIa) pathway to give an active enzyme Factor Xa. Factor Xa as the activator of prothrombin occupies a central position linking the two blood coagulation pathways.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • Tissue specificityPlasma; synthesized in the liver.
  • Involvement in diseaseFactor X deficiency
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Activated factor Xa heavy chain
    • F10
    • FA10_HUMAN
    • Stuart factor
    • Stuart-Prower factor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Factor X Human Chromogenic Activity Assay Kit images

  • Factor X Chromogenic Activity measured in cell culture supernatants showing quantity (ug) per mL of tested sample after 40 min of incubation. Values calculated based on Low Level Standard Curve. Samples were diluted 2-10 fold.

  • Factor X Chromogenic Activity measured in biological fluids showing quantity (ug) per mL of tested sample after 10 min of incubation. Values calculated based on High Level Standard Curve. Samples were diluted 2 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

Protocols

References for Factor X Human Chromogenic Activity Assay Kit (ab108833)

ab108833 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your call last week and for your patience. I apologize for the extended delay in getting this information for you.

I've just received a reply from the lab, and these kits can be analyzed at room temperature. The OD might be slig...

Read More

Thank you for your call today.

As we discussed, you can email mailto:us.sales@abcam.com for information regarding bulk purchases.

Please let me know if you have any questions or if there is anything else that we can do for you.

Thank you for your reply.

Unfortunately we are unable to run inhibitor tests for our customers.

However, if I can help you in any other way please do letme know.

Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you.


The Factor VII Human Chromogenic Activity Assay Kit had been developed to measure human FVII activity in plasma and cell cul...

Read More

Thank you for your recent telephone enquiry. I am sorry to confirm that this kit has not been tested in mouse. Due to the nature of the kit, it is difficult to predict whether it would still work on mouse samples. I am sorry we do not have more informa...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"