Anti-Factor Xa antibody (ab111171)


  • Product nameAnti-Factor Xa antibody
    See all Factor Xa primary antibodies
  • Description
    Rabbit polyclonal to Factor Xa
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native Factor Xa purified from human plasma

  • Positive control
    • ICC/IF: HepG2 cell line.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.50
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab111171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.


  • FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • Tissue specificityPlasma; synthesized in the liver.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activated factor Xa heavy chain antibody
    • Coagulation factor X antibody
    • F10 antibody
    • FA10_HUMAN antibody
    • FX antibody
    • FXA antibody
    • Prothrombinase antibody
    • Stuart factor antibody
    • Stuart Prower factor antibody
    • Stuart-Prower factor antibody
    see all

Anti-Factor Xa antibody images

  • ICC/IF image of ab111171 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111171, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Factor Xa antibody (ab111171)

ab111171 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HDMEC)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification HDMEC
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 28°C

Abcam user community

Verified customer

Submitted Aug 06 2015

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab61389.

To check the status of the order please contact o...

Read More

Thank you for your enquiry.

In the process of setting up your free replacement order for one vial of ab13858, I noticed that the product you called about was ab111171 rather than ab11171, the MDC1 antibody which we discussed. You received the...

Read More