Anti-Factor Xa Heavy Chain antibody (ab75180)

Overview

  • Product nameAnti-Factor Xa Heavy Chain antibody
    See all Factor Xa Heavy Chain primary antibodies
  • Description
    Rabbit polyclonal to Factor Xa Heavy Chain
  • SpecificityDetects endogenous levels of fragment of Factor Xa resulting from cleavage adjacent to Ile235
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from human Factor Xa heavy chain.

  • Positive control
    • Extracts from rat liver cells

Properties

Applications

Our Abpromise guarantee covers the use of ab75180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/20000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 55 kDa (uncleaved precursor).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
    • Tissue specificityPlasma; synthesized in the liver.
    • Involvement in diseaseDefects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
    • Sequence similaritiesBelongs to the peptidase S1 family.
      Contains 2 EGF-like domains.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 1 peptidase S1 domain.
    • Post-translational
      modifications
      The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
      N- and O-glycosylated.
      The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activated factor Xa heavy chain antibody
      • Coagulation factor X antibody
      • F10 antibody
      • FA10_HUMAN antibody
      • Factor X antibody
      • Factor X deficiency antibody
      • Factor Xa antibody
      • FX antibody
      • FXA antibody
      • Prothrombinase antibody
      • Stuart factor antibody
      • Stuart Prower factor antibody
      • Stuart-Prower factor antibody
      • Stuart-Prower factor deficiency antibody
      see all

    Anti-Factor Xa Heavy Chain antibody images

    • All lanes : Anti-Factor Xa Heavy Chain antibody (ab75180) at 1/500 dilution

      Lane 1 : extracts from rat liver cells
      Lane 2 : extracts from rat liver cells with immunizing peptide at 5 µg

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 55 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    References for Anti-Factor Xa Heavy Chain antibody (ab75180)

    ab75180 has not yet been referenced specifically in any publications.

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    I am sorry to confirm that this antibody is regrettably not available without glycerol.

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    The immunogen of ab75180 corresponds to the amino acid sequence between 235-488 (Swiss-port No. P00742). This sequence corresponds to the factor Xa chain of coagulation factor X. This ab will not recognize unclea...

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