Overview

  • Product nameAnti-Factor XII antibody
    See all Factor XII primary antibodies
  • Description
    Goat polyclonal to Factor XII
  • Tested applicationsSuitable for: IP, ELISA, IHC-P, Immunoelectrophoresismore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation - Factor XII purified from Human plasma

  • Positive control
    • Human kidney tissue, Human testis tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 0.15M Sodium chloride, 10mM HEPES, pH 7.4
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoelectrophoresis Use at an assay dependent dilution.

Target

  • FunctionFactor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
  • Involvement in diseaseDefects in F12 are the cause of factor XII deficiency (FA12D) [MIM:234000]; also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection).
    Defects in F12 are the cause of hereditary angioedema type 3 (HAE3) [MIM:610618]; also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g., during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 fibronectin type-I domain.
    Contains 1 fibronectin type-II domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Factor XII is activated by kallikrein in alpha-factor XIIa, which is then further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of the NH2-terminal heavy chain (Coagulation factor XIIa heavy chain) and the COOH-terminal light chain (Coagulation factor XIIa light chain), connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, a light chain (Beta-factor XIIa part 2), corresponding to the COOH-terminal light chain (Coagulation factor XIIa light chain) and a nonapeptide (Beta-factor XIIa part 1).
    O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Factor XII antibody
    • Beta factor XIIa part 1 antibody
    • Beta factor XIIa part 2 antibody
    • Coagulation factor XII antibody
    • Coagulation factor XIIa heavy chain antibody
    • Coagulation factor XIIa light chain antibody
    • F12 antibody
    • F12 deficiency antibody
    • FA12_HUMAN antibody
    • Factor XII deficiency antibody
    • HAE3 antibody
    • HAEX antibody
    • HAF antibody
    • HAF deficiency antibody
    • Hageman factor antibody
    see all

Anti-Factor XII antibody images

  • Human Kidney (formalin-fixed, paraffin-embedded) stained with ab104557 at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human Testis (formalin-fixed, paraffin-embedded) stained with ab104557 at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-Factor XII antibody (ab104557)

ab104557 has not yet been referenced specifically in any publications.

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