Anti-Factor XII heavy chain antibody [B7C9] (ab1007)

Overview

  • Product nameAnti-Factor XII heavy chain antibody [B7C9]
    See all Factor XII heavy chain primary antibodies
  • Description
    Mouse monoclonal [B7C9] to Factor XII heavy chain
  • SpecificityThis antibody reacts with the heavy chain of human Factor XII (mw~50,000) with a KD of 9.8nM.
  • Tested applicationsSuitable for: ELISA, IP, Inhibition Assay, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Purified human Factor XII.

  • EpitopeThe specific epitope for this antibody is located within a 20 amino acid sequence in the surface binding area of Factor XII.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberB7C9
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
Dilute in PBS or medium which is identical to that used in the assay system. This antibody partially blocks Factor XII activity.

Target

  • RelevanceCoagulation factor XII circulates in blood as a zymogen. This single chain zymogen is converted to a two chain serine protease with an heavy chain (alpha factor XIIa) and a light chain. The heavy chain contains two fibronectin type domains, two epidermal growth factor (EGF) like domains, a kringle domain and a proline rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta factor XIIa light chain and the alpha factor XIIa light chain becomes beta factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha factor XIIa and then to beta factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in Factor XII gene do not cause any clinical symptoms and the sole effect is that whole blood clotting time is prolonged.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Coagulation factor XII antibody
    • Coagulation factor XIIa heavy chain antibody
    • F12 antibody
    • HAE3 antibody
    • HAEX antibody
    • HAF antibody
    • Hageman factor antibody
    see all

Anti-Factor XII heavy chain antibody [B7C9] images

References for Anti-Factor XII heavy chain antibody [B7C9] (ab1007)

This product has been referenced in:
  • von Brühl ML  et al. Monocytes, neutrophils, and platelets cooperate to initiate and propagate venous thrombosis in mice in vivo. J Exp Med 209:819-35 (2012). Read more (PubMed: 22451716) »
  • Jablonska E  et al. Transforming growth factor-beta1 induces expression of human coagulation factor XII via Smad3 and JNK signaling pathways in human lung fibroblasts. J Biol Chem 285:11638-51 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20142324) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (Plasma)
Loading amount 4 µg
Specification Plasma
Treatment Kaolin (5mg.ml), Destran (10ug/ml) 37C for 30 min
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 26 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Human)
Loading amount 2 µg
Specification Human
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Feb 12 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"