Anti-Factor XII heavy chain cleaved Arg372 antibody (ab79554)

Overview

  • Product nameAnti-Factor XII heavy chain cleaved Arg372 antibody
  • Description
    Rabbit polyclonal to Factor XII heavy chain cleaved Arg372
  • SpecificityDetects the fragment of activated Factor XII resulting from cleavage adjacent to Arg372.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Extracts from 293 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa.
ELISA 1/20000.

Target

  • FunctionFactor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
  • Involvement in diseaseFactor XII deficiency
    Hereditary angioedema 3
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 fibronectin type-I domain.
    Contains 1 fibronectin type-II domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.
    O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta factor XIIa part 1 antibody
    • Beta factor XIIa part 2 antibody
    • Beta-factor XIIa part 2 antibody
    • Coagulation factor XII antibody
    • Coagulation factor XII (Hageman factor) antibody
    • Coagulation factor XIIa heavy chain antibody
    • Coagulation factor XIIa light chain antibody
    • F12 antibody
    • FA12 antibody
    • FA12_HUMAN antibody
    • HAE3 antibody
    • HAEX antibody
    • HAF antibody
    • Hageman factor antibody
    see all

Anti-Factor XII heavy chain cleaved Arg372 antibody images

  • All lanes : Anti-Factor XII heavy chain cleaved Arg372 antibody (ab79554) at 1/500 dilution

    Lane 1 : Extracts from 293 cells, treated with etoposide (25uM, 1hour)
    Lane 2 : Extracts from 293 cells, treated with etoposide (25uM, 1hour) with immunizing peptide at 5 µg

    Lysates/proteins at 25 µg per lane.


    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-Factor XII heavy chain cleaved Arg372 antibody (ab79554)

ab79554 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"