Overview

  • Product name
    Anti-Factor XIIIa antibody [EP3371]
    See all Factor XIIIa primary antibodies
  • Description
    Rabbit monoclonal [EP3371] to Factor XIIIa
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 75-150 of Human Factor XIIIa

  • Positive control
    • Human placenta, PMBC and rat platelet lysates
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab79759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IP 1/20.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
    • Involvement in disease
      Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
    • Sequence similarities
      Belongs to the transglutaminase superfamily. Transglutaminase family.
    • Post-translational
      modifications
      The activation peptide is released by thrombin.
    • Cellular localization
      Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
      • Coagulation factor XIII A chain antibody
      • Coagulation factor XIII A1 polypeptide antibody
      • Coagulation factor XIII A1 subunit antibody
      • Coagulation factor XIII, A polypeptide antibody
      • Coagulation factor XIIIa antibody
      • F13A antibody
      • F13A_HUMAN antibody
      • F13a1 antibody
      • Factor XIIIA antibody
      • Fibrin stabilizing factor, A subunit antibody
      • Fibrinoligase antibody
      • FSF, A subunit antibody
      • Protein glutamine gamma glutamyltransferase A chain antibody
      • Protein-glutamine gamma-glutamyltransferase A chain antibody
      • TGase antibody
      • Transglutaminase A chain antibody
      • Transglutaminase, plasma antibody
      • Transglutaminase. plasma antibody
      see all

    Images

    • All lanes : Anti-Factor XIIIa antibody [EP3371] (ab79759) at 1/2000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : PMBC lysate
      Lane 3 : Rat platelets lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 83 kDa
      Observed band size : 83 kDa
      Additional bands at : 65 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:
    • Fisher ML  et al. Transglutaminase Is Required for Epidermal Squamous Cell Carcinoma Stem Cell Survival. Mol Cancer Res 13:1083-94 (2015). Read more (PubMed: 25934691) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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