FADD antibody (ab24533)

Product Name 

FADD antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to FADD

Immunogen 

Synthetic peptide: KIDSIEDRYPRNLTERVRESL, corresponding to amino acids 125-145 of Human FADD(Peptide available as ab54083.)
BLAST 'KIDSIEDRYPRNLTERVRESL' with or

Reacts with 
(species key)

Hu, Ms, Rat, Mk

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 4 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Can be blocked with FADD peptide (ab54083).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> FADD
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

Relevance 

FADD (Fas Associated Death Domain) is an apoptosis adapter molecule enabling transduction of the apoptosis signal initiated via the FasL/Fas receptor interaction. The protein contains a C terminal death domain that interacts with the Fas receptor death domain. The N terminus contains a death effectors domain (DED) which recruits caspase to the death inducing signaling complex (DISC) and initiates the apoptotic caspase cascade. Recruitment of Caspase 8 to the Fas receptor results in oligomerization of the Caspase 8 protein, which in turn drives its autoactivation through self-cleavage. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby commiting the cell to undergo apoptosis. FADD is implicated in non-apoptotic cellular pathways such as the regulation of cell cycle machinery in T lymphocytes. This is connected to the phosphorylation state of FADD and to the FasL/TRAIL induced transcriptional activation of cfos protooncogene. FADD also interacts with the hepatitis C virus core protein in the HEK 293 cell line.

FADD has a molecular weight of 23kDa with an apparent molecular weight of 28 kDa on SDS-PAGE.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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