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KIDSIEDRYPRNLTERVRESL, corresponding to amino acids 125-145 of Human FADD (Peptide available as ab54083.)
FADD has a molecular weight of 23kDa with an apparent molecular weight of 28 kDa on SDS-PAGE.
Our Abpromise guarantee covers the use of ab24533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).Can be blocked with FADD peptide (ab54083).|
|IP||Use at an assay dependent concentration.|