Overview

  • Product nameAnti-FADD antibody
    See all FADD primary antibodies
  • Description
    Rabbit polyclonal to FADD
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide:

    KIDSIEDRYPRNLTERVRESL

    , corresponding to amino acids 125-145 of Human FADD (Peptide available as ab54083.)

  • General notes


    FADD has a molecular weight of 23kDa with an apparent molecular weight of 28 kDa on SDS-PAGE.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab24533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).Can be blocked with FADD peptide (ab54083).
IP Use at an assay dependent concentration.

Target

  • FunctionApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
  • Tissue specificityExpressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
  • Sequence similaritiesContains 1 death domain.
    Contains 1 DED (death effector) domain.
  • DomainContains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • FADD antibody
    • FADD protein antibody
    • FADD_HUMAN antibody
    • Fas (TNFRSF6) associated via death domain antibody
    • Fas associated via death domain antibody
    • Fas associating death domain containing protein antibody
    • Fas associating protein antibody
    • Fas associating protein with death domain antibody
    • Fas TNFRSF6 associated via death domain antibody
    • FAS-associated death domain protein antibody
    • FAS-associating death domain-containing protein antibody
    • GIG 3 antibody
    • GIG3 antibody
    • Growth inhibiting gene 3 protein antibody
    • Growth-inhibiting gene 3 protein antibody
    • H sapiens mRNA for mediator of receptor induced toxicity antibody
    • Mediator of receptor induced toxicity antibody
    • MGC8528 antibody
    • MORT 1 antibody
    • MORT1 antibody
    • Protein FADD antibody
    see all

Anti-FADD antibody images

  • ICC/IF image of ab24533 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24533, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab24533 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24533, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-FADD antibody (ab24533)

This product has been referenced in:
  • Seko Y  et al. Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci Rep 5:13737 (2015). Read more (PubMed: 26348594) »
  • Jackson R  et al. Paracrine Engineering of Human Cardiac Stem Cells With Insulin-Like Growth Factor 1 Enhances Myocardial Repair. J Am Heart Assoc 4:e002104 (2015). WB . Read more (PubMed: 26363004) »

See all 3 Publications for this product

Product Wall

Thank you for your patience in this matter. I have received a reply from the originator of ab24533 (FADD antibody). The immunogenic peptide sequence used in raising this product is: KIDSIEDRYPRNLTERVRESL. Please do not hesitate to contact us a...

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Thank you for your recent enquiry. I have been informed that the immunogen sequence is within the range of amino acids 100-150 of human FADD, I am unable to provide the specific immunogen sequence as this information is confidential. I hope this inf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"