The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
1/10 - 1/100.
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Use at an assay dependent concentration.
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Contains 1 death domain. Contains 1 DED (death effector) domain.
Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
Fas (TNFRSF6) associated via death domain antibody
Fas associated via death domain antibody
Fas associating death domain containing protein antibody
Fas associating protein antibody
Fas associating protein with death domain antibody
Fas TNFRSF6 associated via death domain antibody
FAS-associated death domain protein antibody
FAS-associating death domain-containing protein antibody
GIG 3 antibody
Growth inhibiting gene 3 protein antibody
Growth-inhibiting gene 3 protein antibody
H sapiens mRNA for mediator of receptor induced toxicity antibody
Mediator of receptor induced toxicity antibody
MORT 1 antibody
Protein FADD antibody
Western blot - Anti-FADD antibody [EPR4415] (ab108601)
Predicted band size : 23 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: FADD knockout HAP1 cell lysate (20 µg) Lane 3: A431 cell lysate (20 µg) Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108601 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa. ab108601 was shown to specifically react with FADD when FADD knockout samples were used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab108601 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Flow Cytometry analysis of A431(human epidermoid carcinoma) cells labeling FADD with purified ab108601 at 1/140 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
Western blot - FADD antibody [EPR4415] (ab108601)
All lanes : Anti-FADD antibody [EPR4415] (ab108601) at 1/1000 dilution
Lane 1 : A431 cell lysate Lane 2 : Jurkat cell lysate Lane 3 : HeLa cell lysate Lane 4 : SKBR-3 cell lysate
Liu Y et al. MiR-7a is an important mediator in Fas-associated protein with death domain (FADD)-regulated expression of focal adhesion kinase (FAK). Oncotarget7:51393-51407 (2016).
Read more (PubMed: 27286445) »
Lu YM et al. P2X7 signaling promotes microsphere embolism-triggered microglia activation by maintaining elevation of Fas ligand. J Neuroinflammation9:172 (2012).
Read more (PubMed: 22789015) »