Overview

  • Product nameAnti-FADD antibody [EPR5030]
    See all FADD primary antibodies
  • Description
    Rabbit monoclonal [EPR5030] to FADD
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Mouse FADD.

  • Positive control
    • Mouse kidney tissue; Mouse spleen lysate, Raw264.7 cell lysate and NIH/3T3 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab124812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
  • Application notesIs unsuitable for ICC or IP.
  • Target

    • FunctionApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
    • Tissue specificityExpressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
    • Sequence similaritiesContains 1 death domain.
      Contains 1 DED (death effector) domain.
    • DomainContains a death domain involved in the binding of the corresponding domain within Fas receptor.
    • Information by UniProt
    • Database links
    • Alternative names
      • FADD antibody
      • FADD protein antibody
      • FADD_HUMAN antibody
      • Fas (TNFRSF6) associated via death domain antibody
      • Fas associated via death domain antibody
      • Fas associating death domain containing protein antibody
      • Fas associating protein antibody
      • Fas associating protein with death domain antibody
      • Fas TNFRSF6 associated via death domain antibody
      • FAS-associated death domain protein antibody
      • FAS-associating death domain-containing protein antibody
      • GIG 3 antibody
      • GIG3 antibody
      • Growth inhibiting gene 3 protein antibody
      • Growth-inhibiting gene 3 protein antibody
      • H sapiens mRNA for mediator of receptor induced toxicity antibody
      • Mediator of receptor induced toxicity antibody
      • MGC8528 antibody
      • MORT 1 antibody
      • MORT1 antibody
      • Protein FADD antibody
      see all

    Anti-FADD antibody [EPR5030] images

    • All lanes : Anti-FADD antibody [EPR5030] (ab124812) at 1/1000 dilution

      Lane 1 : Mouse spleen lysate
      Lane 2 : Raw264.7 cell lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 23 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    • ab124812, at 1/50 dilution, staining FADD in paraffin-embedded Mouse Kidney tissue by Immunohistochemistry.

    References for Anti-FADD antibody [EPR5030] (ab124812)

    This product has been referenced in:
    • Jaiswal MK  et al. Regulation of apoptosis and innate immune stimuli in inflammation-induced preterm labor. J Immunol 191:5702-13 (2013). WB, IHC-Fr ; Mouse . Read more (PubMed: 24163412) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
    Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount 500000 cells
    Specification Canine Kidney Epithelial MDCK cells
    Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 11 2016

    Application Immunoprecipitation
    Sample Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
    Total protein in input 5e+006 cells
    Treatment 50 ng/mL TNFalpha for 5 hours
    Immuno-precipitation step Other - Protein G Dynabeads
    Specification mouse embryonic fibroblasts
    Username

    Abcam user community

    Verified customer

    Submitted May 03 2016

    Application Western blot
    Sample Human Cell lysate - whole cell (Human Foreskin Fibroblasts & Mouse embryonic fibro)
    Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
    Loading amount 200000 cells
    Specification Human Foreskin Fibroblasts & Mouse embryonic fibro
    Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 05 2016

    Application Western blot
    Sample Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
    Gel Running Conditions Reduced Denaturing
    Loading amount 10000 cells
    Treatment With or without 100uM zVAD
    Specification mouse embryonic fibroblasts
    Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Username

    Dr. Yi-Chieh Perng

    Verified customer

    Submitted Nov 30 2015

    Application Western blot
    Loading amount 5 µg
    Gel Running Conditions Reduced Denaturing (4-12)
    Sample Mouse Tissue lysate - whole (uterus)
    Specification uterus
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Varkha Agrawal

    Verified customer

    Submitted Jan 07 2014

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample Mouse Tissue sections (Uterus)
    Specification Uterus
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Dr. Mukesh Jaiswal

    Verified customer

    Submitted Dec 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"