Recombinant
RabMAb

Anti-FADD antibody [EPR5030] (ab124812)

Overview

  • Product name
    Anti-FADD antibody [EPR5030]
    See all FADD primary antibodies
  • Description
    Rabbit monoclonal [EPR5030] to FADD
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Mouse FADD.

  • Positive control
    • Mouse kidney tissue; Mouse spleen lysate, Raw264.7 cell lysate and NIH/3T3 cell lysate.
  • General notes

     

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
    • Tissue specificity
      Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
    • Sequence similarities
      Contains 1 death domain.
      Contains 1 DED (death effector) domain.
    • Domain
      Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
    • Information by UniProt
    • Database links
    • Alternative names
      • FADD antibody
      • FADD protein antibody
      • FADD_HUMAN antibody
      • Fas (TNFRSF6) associated via death domain antibody
      • Fas associated via death domain antibody
      • Fas associating death domain containing protein antibody
      • Fas associating protein antibody
      • Fas associating protein with death domain antibody
      • Fas TNFRSF6 associated via death domain antibody
      • FAS-associated death domain protein antibody
      • FAS-associating death domain-containing protein antibody
      • GIG 3 antibody
      • GIG3 antibody
      • Growth inhibiting gene 3 protein antibody
      • Growth-inhibiting gene 3 protein antibody
      • H sapiens mRNA for mediator of receptor induced toxicity antibody
      • Mediator of receptor induced toxicity antibody
      • MGC8528 antibody
      • MORT 1 antibody
      • MORT1 antibody
      • Protein FADD antibody
      see all

    Anti-FADD antibody [EPR5030] images

    • All lanes : Anti-FADD antibody [EPR5030] (ab124812) at 1/1000 dilution

      Lane 1 : Mouse spleen lysate
      Lane 2 : Raw264.7 cell lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 23 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    • ab124812, at 1/50 dilution, staining FADD in paraffin-embedded Mouse Kidney tissue by Immunohistochemistry.

    References for Anti-FADD antibody [EPR5030] (ab124812)

    This product has been referenced in:
    • Bowman BM  et al. Phosphorylation of FADD by the kinase CK1a promotes KRASG12D-induced lung cancer. Sci Signal 8:ra9 (2015). Read more (PubMed: 25628462) »
    • Jaiswal MK  et al. Regulation of apoptosis and innate immune stimuli in inflammation-induced preterm labor. J Immunol 191:5702-13 (2013). WB, IHC-Fr ; Mouse . Read more (PubMed: 24163412) »

    See all 2 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    0.5 µg
    Specification
    Rabbit Reticulocyte
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 09 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (BHK-21 (Kidney Fibroblasts))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MOPS buffer))
    Loading amount
    1e+006 cells
    Specification
    BHK-21 (Kidney Fibroblasts)
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 08 2017

    Application
    Western blot
    Sample
    Chinese Hamster Cell lysate - whole cell (Chinese hamster ovary (CHO) cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
    Loading amount
    50000 cells
    Specification
    Chinese hamster ovary (CHO) cells
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 12 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    500000 cells
    Specification
    Canine Kidney Epithelial MDCK cells
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 11 2016

    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
    Total protein in input
    5e+006 cells
    Treatment
    50 ng/mL TNFalpha for 5 hours
    Immuno-precipitation step
    Other - Protein G Dynabeads
    Specification
    mouse embryonic fibroblasts
    Username

    Abcam user community

    Verified customer

    Submitted May 03 2016

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Foreskin Fibroblasts & Mouse embryonic fibro)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
    Loading amount
    200000 cells
    Specification
    Human Foreskin Fibroblasts & Mouse embryonic fibro
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 05 2016

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10000 cells
    Treatment
    With or without 100uM zVAD
    Specification
    mouse embryonic fibroblasts
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Username

    Dr. Yi-Chieh Perng

    Verified customer

    Submitted Nov 30 2015

    Application
    Western blot
    Loading amount
    5 µg
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Sample
    Mouse Tissue lysate - whole (uterus)
    Specification
    uterus
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Varkha Agrawal

    Verified customer

    Submitted Jan 07 2014

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample
    Mouse Tissue sections (Uterus)
    Specification
    Uterus
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Dr. Mukesh Jaiswal

    Verified customer

    Submitted Dec 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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