Recombinant
RabMAb

Anti-FADD antibody [EPR5030] (ab124812)

Abreviews (9) Submit a Question Specific References (3)

Overview

  • Product name
    Anti-FADD antibody [EPR5030]
    See all FADD primary antibodies
  • Description
    Rabbit monoclonal [EPR5030] to FADD
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Mouse FADD.

  • Positive control
    • Mouse kidney tissue; Mouse spleen lysate, Raw264.7 cell lysate and NIH/3T3 cell lysate.
  • General notes

     

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function
      Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
    • Tissue specificity
      Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
    • Sequence similarities
      Contains 1 death domain.
      Contains 1 DED (death effector) domain.
    • Domain
      Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
    • Information by UniProt
    • Database links
      • Alternative names
        • FADD antibody
        • FADD protein antibody
        • FADD_HUMAN antibody
        • Fas (TNFRSF6) associated via death domain antibody
        • Fas associated via death domain antibody
        • Fas associating death domain containing protein antibody
        • Fas associating protein antibody
        • Fas associating protein with death domain antibody
        • Fas TNFRSF6 associated via death domain antibody
        • FAS-associated death domain protein antibody
        • FAS-associating death domain-containing protein antibody
        • GIG 3 antibody
        • GIG3 antibody
        • Growth inhibiting gene 3 protein antibody
        • Growth-inhibiting gene 3 protein antibody
        • H sapiens mRNA for mediator of receptor induced toxicity antibody
        • Mediator of receptor induced toxicity antibody
        • MGC8528 antibody
        • MORT 1 antibody
        • MORT1 antibody
        • Protein FADD antibody
        see all

      Images

      • Western blot - Anti-FADD antibody [EPR5030] (ab124812)
        Western blot - Anti-FADD antibody [EPR5030] (ab124812)
        All lanes : Anti-FADD antibody [EPR5030] (ab124812) at 1/1000 dilution

        Lane 1 : Mouse spleen lysate
        Lane 2 : Raw264.7 cell lysate
        Lane 3 : NIH/3T3 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 23 kDa
        Observed band size: 28 kDa (why is the actual band size different from the predicted?)

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] (ab124812)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] (ab124812)
        ab124812, at 1/50 dilution, staining FADD in paraffin-embedded Mouse Kidney tissue by Immunohistochemistry.

      References

      This product has been referenced in:
      • Geng J  et al. Regulation of RIPK1 activation by TAK1-mediated phosphorylation dictates apoptosis and necroptosis. Nat Commun 8:359 (2017). Read more (PubMed: 28842570) »
      • Bowman BM  et al. Phosphorylation of FADD by the kinase CK1a promotes KRASG12D-induced lung cancer. Sci Signal 8:ra9 (2015). Read more (PubMed: 25628462) »

      See all 3 Publications for this product

      Publishing research using ab124812? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      9 reviews or Q&A

      10
      Votes: Highest First

      Western blot abreview for Anti-FADD antibody [EPR5030]

       Good
      Abreviews
      Application
      Western blot
      Sample
      Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
      Loading amount
      0.5 µg
      Specification
      Rabbit Reticulocyte
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More
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      Username

      Abcam user community

      Verified customer

      Submitted Feb 09 2017

      Western blot abreview for Anti-FADD antibody [EPR5030]

       Excellent
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Western blot
      Sample
      Hamster Cell lysate - whole cell (BHK-21 (Kidney Fibroblasts))
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MOPS buffer))
      Loading amount
      1e+006 cells
      Specification
      BHK-21 (Kidney Fibroblasts)
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Feb 08 2017

      Western blot abreview for Anti-FADD antibody [EPR5030]

       Average
      Abreviews
      Application
      Western blot
      Sample
      Chinese Hamster Cell lysate - whole cell (Chinese hamster ovary (CHO) cells)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
      Loading amount
      50000 cells
      Specification
      Chinese hamster ovary (CHO) cells
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Dec 12 2016

      Western blot abreview for Anti-FADD antibody [EPR5030]

       Inconclusive
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Western blot
      Sample
      Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
      Loading amount
      500000 cells
      Specification
      Canine Kidney Epithelial MDCK cells
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Nov 11 2016

      Immunoprecipitation abreview for Anti-FADD antibody [EPR5030]

       Good
      Abreviews
      Application
      Immunoprecipitation
      Sample
      Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
      Total protein in input
      5e+006 cells
      Treatment
      50 ng/mL TNFalpha for 5 hours
      Immuno-precipitation step
      Other - Protein G Dynabeads
      Specification
      mouse embryonic fibroblasts
      Read More
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      Username

      Abcam user community

      Verified customer

      Submitted May 03 2016

      Western blot abreview for Anti-FADD antibody [EPR5030]

       Excellent
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human Foreskin Fibroblasts & Mouse embryonic fibro)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
      Loading amount
      200000 cells
      Specification
      Human Foreskin Fibroblasts & Mouse embryonic fibro
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Feb 05 2016

      Western blot abreview for Anti-FADD antibody [EPR5030]

       Good
      Abreviews
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      10000 cells
      Treatment
      With or without 100uM zVAD
      Specification
      mouse embryonic fibroblasts
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
      Read More
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      Username

      Dr. Yi-Chieh Perng

      Verified customer

      Submitted Nov 30 2015

      Western blot abreview for Anti-FADD antibody [EPR5030]

       Excellent
      Abreviews
      Application
      Western blot
      Loading amount
      5 µg
      Gel Running Conditions
      Reduced Denaturing (4-12)
      Sample
      Mouse Tissue lysate - whole (uterus)
      Specification
      uterus
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
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      Username

      Dr. Varkha Agrawal

      Verified customer

      Submitted Jan 07 2014

      Immunohistochemistry (Frozen sections) abreview for Anti-FADD antibody [EPR5030]

       Excellent
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
      Sample
      Mouse Tissue sections (Uterus)
      Specification
      Uterus
      Permeabilization
      No
      Fixative
      Paraformaldehyde
      Read More
      abreview image
      Username

      Dr. Mukesh Jaiswal

      Verified customer

      Submitted Dec 04 2013

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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