Recombinant
RabMAb

Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)

Overview

  • Product name
    Anti-FADD antibody [EPR5030] - BSA and Azide free
    See all FADD primary antibodies
  • Description
    Rabbit monoclonal [EPR5030] to FADD - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Mouse FADD.

  • Positive control
    • Mouse kidney tissue; Mouse spleen lysate, Raw264.7 cell lysate and NIH/3T3 cell lysate.
  • General notes

    ab229444 is a PBS-only buffer formulated version of ab124812, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab124812 for information on protocols, dilutions, and image data.

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab229444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
    • Tissue specificity
      Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
    • Sequence similarities
      Contains 1 death domain.
      Contains 1 DED (death effector) domain.
    • Domain
      Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
    • Information by UniProt
    • Database links
    • Alternative names
      • FADD antibody
      • FADD protein antibody
      • FADD_HUMAN antibody
      • Fas (TNFRSF6) associated via death domain antibody
      • Fas associated via death domain antibody
      • Fas associating death domain containing protein antibody
      • Fas associating protein antibody
      • Fas associating protein with death domain antibody
      • Fas TNFRSF6 associated via death domain antibody
      • FAS-associated death domain protein antibody
      • FAS-associating death domain-containing protein antibody
      • GIG 3 antibody
      • GIG3 antibody
      • Growth inhibiting gene 3 protein antibody
      • Growth-inhibiting gene 3 protein antibody
      • H sapiens mRNA for mediator of receptor induced toxicity antibody
      • Mediator of receptor induced toxicity antibody
      • MGC8528 antibody
      • MORT 1 antibody
      • MORT1 antibody
      • Protein FADD antibody
      see all

    Images

    • This IHC data was generated using the same anti-FADD antibody clone, EPR5030, in a different buffer formulation (cat# ab124812).

      ab124812, at 1/50 dilution, staining FADD in paraffin-embedded Mouse Kidney tissue by Immunohistochemistry.

    References

    ab229444 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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