Anti-FADD (phospho S191) antibody (ab111379)

Overview

  • Product name
    Anti-FADD (phospho S191) antibody
    See all FADD primary antibodies
  • Description
    Rabbit polyclonal to FADD (phospho S191)
  • Specificity
    ab111379 detects endogenous levels of FADD only when phosphorylated at serine 191.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse FADD (C terminal) (phospho S191).
    Sequence:

    NMSPV

  • Positive control
    • Human breast carcinoma tissue; Jurkat cell extracts, treated with PMA (125ng/ml, 30mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab111379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
  • Tissue specificity
    Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
  • Sequence similarities
    Contains 1 death domain.
    Contains 1 DED (death effector) domain.
  • Domain
    Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • FADD antibody
    • FADD protein antibody
    • FADD_HUMAN antibody
    • Fas (TNFRSF6) associated via death domain antibody
    • Fas associated via death domain antibody
    • Fas associating death domain containing protein antibody
    • Fas associating protein antibody
    • Fas associating protein with death domain antibody
    • Fas TNFRSF6 associated via death domain antibody
    • FAS-associated death domain protein antibody
    • FAS-associating death domain-containing protein antibody
    • GIG 3 antibody
    • GIG3 antibody
    • Growth inhibiting gene 3 protein antibody
    • Growth-inhibiting gene 3 protein antibody
    • H sapiens mRNA for mediator of receptor induced toxicity antibody
    • Mediator of receptor induced toxicity antibody
    • MGC8528 antibody
    • MORT 1 antibody
    • MORT1 antibody
    • Protein FADD antibody
    see all

Images

  • All lanes : Anti-FADD (phospho S191) antibody (ab111379) at 1/500 dilution

    Lane 1 : Jurkat cell extracts, treated with PMA (125ng/ml, 30mins)
    Lane 2 : Jurkat cell extracts, treated with PMA (125ng/ml, 30mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 23 kDa
  • ab111379 at 1/50 dilution staining FADD in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

References

ab111379 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (Chinese hamster ovary (CHO) cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MOPS buffer))
Loading amount
50000 cells
Specification
Chinese hamster ovary (CHO) cells
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MOPS buffer))
Loading amount
25000 cells
Specification
Canine Kidney Epithelial MDCK cells
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (VERO E6)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MOPS buffer))
Loading amount
75000 cells
Specification
VERO E6
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 17 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Human Foreskin Fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
Loading amount
200000 cells
Specification
Human Foreskin Fibroblasts
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 19 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic fibroblasts (MEFs))
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
Loading amount
200000 cells
Specification
mouse embryonic fibroblasts (MEFs)
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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