Anti-FADD (phospho S194) antibody (ab55334)

Overview

  • Product name
    Anti-FADD (phospho S194) antibody
    See all FADD primary antibodies
  • Description
    Rabbit polyclonal to FADD (phospho S194)
  • Specificity
    This antibody detects endogenous levels of FADD only when phosphorylated at serine 194.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human FADD around the phosphorylation site of serine 194 (A-M-SP-P-M).

  • Positive control
    • Extracts from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab55334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function
    Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
  • Tissue specificity
    Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
  • Sequence similarities
    Contains 1 death domain.
    Contains 1 DED (death effector) domain.
  • Domain
    Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Information by UniProt
  • Database links
  • Alternative names
    • FADD antibody
    • FADD protein antibody
    • FADD_HUMAN antibody
    • Fas (TNFRSF6) associated via death domain antibody
    • Fas associated via death domain antibody
    • Fas associating death domain containing protein antibody
    • Fas associating protein antibody
    • Fas associating protein with death domain antibody
    • Fas TNFRSF6 associated via death domain antibody
    • FAS-associated death domain protein antibody
    • FAS-associating death domain-containing protein antibody
    • GIG 3 antibody
    • GIG3 antibody
    • Growth inhibiting gene 3 protein antibody
    • Growth-inhibiting gene 3 protein antibody
    • H sapiens mRNA for mediator of receptor induced toxicity antibody
    • Mediator of receptor induced toxicity antibody
    • MGC8528 antibody
    • MORT 1 antibody
    • MORT1 antibody
    • Protein FADD antibody
    see all

Anti-FADD (phospho S194) antibody images

  • All lanes : Anti-FADD (phospho S194) antibody (ab55334) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells treated with Paclitaxel (1µM, 60mins).
    Lane 2 : Extracts from HeLa cells treated with Paclitaxel (1µM, 60mins), and with the immunising phosphpeptide.


    Predicted band size : 23 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-FADD (phospho S194) antibody (ab55334)

ab55334 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
Loading amount
0.5 µg
Specification
Rabbit Reticulocyte
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Dec 01 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - whole cell (BHK-21 (Kidney Fibroblasts))
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
500000 cells
Specification
BHK-21 (Kidney Fibroblasts)
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Oct 31 2016

Application
Western blot
Sample
Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
500000 cells
Specification
Canine Kidney Epithelial MDCK cells
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Oct 21 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (Human Foreskin Fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
Loading amount
200000 cells
Specification
Human Foreskin Fibroblasts
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Feb 09 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic fibroblasts (MEFs))
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
Loading amount
200000 cells
Specification
mouse embryonic fibroblasts (MEFs)
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Feb 09 2016

I am very pleased to hear you would like to accept our offer and test ab55334 on mouse samples by western blotting. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview of mouse r...

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If we know of our antibodies being used in any references/publications, we will list these under the "Specific References" section of our datasheet, which can be found under the shipping and pricing...

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