Recombinant
RabMAb

Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)

Overview

  • Product name
    Anti-FADD (phospho S194) antibody [EPR1816Y]
    See all FADD primary antibodies
  • Description
    Rabbit monoclonal [EPR1816Y] to FADD (phospho S194)
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding serine 194 of human FADD.

  • Positive control
    • Jurkat cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab68475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: 1/30.
    WB: 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).

    Is unsuitable for Flow Cyt, ICC/IF or IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
    • Tissue specificity
      Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
    • Sequence similarities
      Contains 1 death domain.
      Contains 1 DED (death effector) domain.
    • Domain
      Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
    • Information by UniProt
    • Database links
    • Alternative names
      • FADD antibody
      • FADD protein antibody
      • FADD_HUMAN antibody
      • Fas (TNFRSF6) associated via death domain antibody
      • Fas associated via death domain antibody
      • Fas associating death domain containing protein antibody
      • Fas associating protein antibody
      • Fas associating protein with death domain antibody
      • Fas TNFRSF6 associated via death domain antibody
      • FAS-associated death domain protein antibody
      • FAS-associating death domain-containing protein antibody
      • GIG 3 antibody
      • GIG3 antibody
      • Growth inhibiting gene 3 protein antibody
      • Growth-inhibiting gene 3 protein antibody
      • H sapiens mRNA for mediator of receptor induced toxicity antibody
      • Mediator of receptor induced toxicity antibody
      • MGC8528 antibody
      • MORT 1 antibody
      • MORT1 antibody
      • Protein FADD antibody
      see all

    Images

    • All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution

      Lane 1 : Jurkat cell lysate, untreated.
      Lane 2 : Jurkat cell lysate, treated with calyculin A.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 23 kDa
      Observed band size : 27 kDa (why is the actual band size different from the predicted?)

    References

    ab68475 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab68475.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up