Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)


  • Product nameAnti-FADD (phospho S194) antibody [EPR1816Y]
    See all FADD primary antibodies
  • Description
    Rabbit monoclonal [EPR1816Y] to FADD (phospho S194)
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding serine 194 of human FADD.

  • Positive control
    • Jurkat cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab68475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: 1/30.
    WB: 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).

    Is unsuitable for Flow Cyt, ICC/IF or IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
    • Tissue specificityExpressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
    • Sequence similaritiesContains 1 death domain.
      Contains 1 DED (death effector) domain.
    • DomainContains a death domain involved in the binding of the corresponding domain within Fas receptor.
    • Information by UniProt
    • Database links
    • Alternative names
      • FADD antibody
      • FADD protein antibody
      • FADD_HUMAN antibody
      • Fas (TNFRSF6) associated via death domain antibody
      • Fas associated via death domain antibody
      • Fas associating death domain containing protein antibody
      • Fas associating protein antibody
      • Fas associating protein with death domain antibody
      • Fas TNFRSF6 associated via death domain antibody
      • FAS-associated death domain protein antibody
      • FAS-associating death domain-containing protein antibody
      • GIG 3 antibody
      • GIG3 antibody
      • Growth inhibiting gene 3 protein antibody
      • Growth-inhibiting gene 3 protein antibody
      • H sapiens mRNA for mediator of receptor induced toxicity antibody
      • Mediator of receptor induced toxicity antibody
      • MGC8528 antibody
      • MORT 1 antibody
      • MORT1 antibody
      • Protein FADD antibody
      see all

    Anti-FADD (phospho S194) antibody [EPR1816Y] images

    • All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution

      Lane 1 : Jurkat cell lysate, untreated.
      Lane 2 : Jurkat cell lysate, treated with calyculin A.

      Lysates/proteins at 10 µg per lane.

      HRP conjugated Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 23 kDa
      Observed band size : 27 kDa (why is the actual band size different from the predicted?)

    References for Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)

    ab68475 has not yet been referenced specifically in any publications.

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