The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: 1/30.
WB: 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).
Is unsuitable for Flow Cyt, ICC/IF or IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Tissue specificityExpressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.