Overview

  • Product nameAnti-FADS1 antibody
    See all FADS1 primary antibodies
  • Description
    Mouse monoclonal to FADS1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
    Predicted to work with: Pig
  • Immunogen

    Recombinant fragment: MAPDPVAAET AAQGPTPRYF TWDEVAQRSG CEERWLVIDR KVYNISEFTR RHPGGSRVIS HYAGQDATDP FVAFHINKGL VKKYMNSLLI GELSPEQPS, corresponding to amino acids 1-99 of Human FADS1 (NP_037534) with a 26kDa tag.

  • Positive control
    • HeLa nuclear extract

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA Use at an assay dependent dilution. Detection limit is approximately 0.03ng/ml as a capture antibody when used against the immunogen.

Target

  • FunctionComponent of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively.
  • Tissue specificityExpressed in many tissues, it is most abundant in the liver, brain, adrenal gland and heart. Found as well in skeletal muscle, lung, placenta, kidney, pancreas and retina.
  • PathwayLipid metabolism; polyunsaturated fatty acid biosynthesis.
  • Sequence similaritiesBelongs to the fatty acid desaturase family.
    Contains 1 cytochrome b5 heme-binding domain.
  • Developmental stageHighly expressed in fetal liver and fetal brain.
  • DomainThe histidine box domains may contain the active site and/or be involved in metal ion binding.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • D5D antibody
    • Delta 5 desaturase antibody
    • Delta 5 fatty acid desaturase antibody
    • Delta(5) desaturase antibody
    • Delta(5) fatty acid desaturase antibody
    • Delta-5 desaturase antibody
    • Fads1 antibody
    • FADS1_HUMAN antibody
    • FADS6 antibody
    • FADSD5 antibody
    • Fatty acid desaturase 1 antibody
    • FLJ38956 antibody
    • Linoleoyl CoA desaturase (delta 6 desaturase) like 1 antibody
    • LLCDL1 antibody
    • TU12 antibody
    see all

Anti-FADS1 antibody images

  • Anti-FADS1 antibody (ab77312) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 52 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-FADS1 antibody (ab77312) at 5 µg/ml + HeLa nuclear extract at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Detection limit for recombinant tagged FADS1 is approximately 0.03ng/ml as a capture antibody

References for Anti-FADS1 antibody (ab77312)

ab77312 has not yet been referenced specifically in any publications.

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