Overview

  • Product name
    Anti-FADS1 antibody [EPR6898]
    See all FADS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6898] to FADS1
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human FADS1.

  • Positive control
    • HepG2, Human fetal lung, fetal liver and fetal brain lysates; Human heart tissue; A549 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/100 - 1/250.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively.
    • Tissue specificity
      Expressed in many tissues, it is most abundant in the liver, brain, adrenal gland and heart. Found as well in skeletal muscle, lung, placenta, kidney, pancreas and retina.
    • Pathway
      Lipid metabolism; polyunsaturated fatty acid biosynthesis.
    • Sequence similarities
      Belongs to the fatty acid desaturase family.
      Contains 1 cytochrome b5 heme-binding domain.
    • Developmental stage
      Highly expressed in fetal liver and fetal brain.
    • Domain
      The histidine box domains may contain the active site and/or be involved in metal ion binding.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • D5D antibody
      • Delta 5 desaturase antibody
      • Delta 5 fatty acid desaturase antibody
      • Delta(5) desaturase antibody
      • Delta(5) fatty acid desaturase antibody
      • Delta-5 desaturase antibody
      • Fads1 antibody
      • FADS1_HUMAN antibody
      • FADS6 antibody
      • FADSD5 antibody
      • Fatty acid desaturase 1 antibody
      • FLJ38956 antibody
      • Linoleoyl CoA desaturase (delta 6 desaturase) like 1 antibody
      • LLCDL1 antibody
      • TU12 antibody
      see all

    Images

    • Overlay histogram showing HepG2 cells stained with ab126706 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126706, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-FADS1 antibody [EPR6898] (ab126706) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : Human fetal brain lysate
      Lane 4 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 52 kDa
    • ab126706, at 1/100 dilution, staining FADS1 in Paraffin-embedded Human heart tissue by Immunohistochemistry.
    • ab126706, at 1/100 dilution, staining FADS1 in A549 cells by Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Pan G  et al. PATZ1 down-regulates FADS1 by binding to rs174557 and is opposed by SP1/SREBP1c. Nucleic Acids Res 45:2408-2422 (2017). WB . Read more (PubMed: 27932482) »
    • Muir K  et al. Proteomic and lipidomic signatures of lipid metabolism in NASH-associated hepatocellular carcinoma. Cancer Res 73:4722-31 (2013). Read more (PubMed: 23749645) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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