The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
Use a concentration of 3 µg/ml.
May play a role in the immune system processes. Protects cells from FAS-, TNF alpha- and FADD-induced apoptosis without increasing expression of the inhibitors of apoptosis BCL2 and BCLXL. Seems to activate an inhibitory pathway that prevents CASP8 activation following FAS stimulation, rather than blocking apoptotic signals downstream. May inhibit FAS-induced apoptosis by preventing CASP8 processing through CFLAR up-regulation.
Expressed in lymph nodes, peripheral blood leukocytes, lung, thymus and kidneys. Very weak expression detected in spleen, liver, heart, and salivary gland. Expressed in lymphoid cell lines such as Jurkat cells, CemT4 cells, MolT-4 cells, HB11.19 cells, and Reh cells. No expression detected in nonhematopoietic cell lines, including HepG2 cells, 293 cells and Hela cells.
Contains 1 Ig-like (immunoglobulin-like) domain.
The Ig-like domain is required for the anti-apoptotic ability.